Comparative responses of cattle, sheep, and goats to larvae of the emerging trematode, Dicrocoelium dendriticum, and development of a diagnostic tool

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dc.contributor.authorPhillips, Natalia D.
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorGoater, Cameron P.
dc.contributor.supervisorColwell, Douglas
dc.date.accessioned2016-05-18T22:06:04Z
dc.date.available2016-05-18T22:06:04Z
dc.date.issued2016
dc.degree.levelMastersen_US
dc.description.abstractParasitic infections caused by species of gastrointestinal ‘worms’ constrain efficient livestock production through the negative effects they have on individual hosts. Dicrocoelium dendriticum is a host generalist trematode that infects grazing mammals and is an introduced species in Alberta. Reducing the impact of emerging, generalist parasites such as this one requires sensitive and specific diagnosis of individual hosts as well as an understanding of relative host responses to infection. I found high interspecific variation in fluke intensity, fluke recruitment, fecal egg counts, and antibody responses in experimentally infected cattle, sheep, and goats. Fluke intensity was significantly higher in goats (x=257±38) than in sheep (x=132±80) or cattle (x=111±103). Anti-fluke IgG and IgM antibody concentrations were high in sheep sera, but undetectable in cattle sera. A coproantigen ELISA detected D. dendriticum protein in cattle and sheep feces at 9 days post-infection. However, assay sensitivities were poor in sheep (x=45%) and cattle (x=23%).en_US
dc.embargoNoen_US
dc.identifier.urihttps://hdl.handle.net/10133/4480
dc.language.isoen_CAen_US
dc.proquest.subject0718en_US
dc.proquest.subject0778en_US
dc.proquest.subject0982en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta : University of Lethbridge, Dept. of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectcoproantigensen_US
dc.subjectELISAen_US
dc.subjectenzyme-linked immunosorbent assayen_US
dc.subjecthost generalist parasitesen_US
dc.subjecthost immune responseen_US
dc.titleComparative responses of cattle, sheep, and goats to larvae of the emerging trematode, Dicrocoelium dendriticum, and development of a diagnostic toolen_US
dc.typeThesisen_US
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