Rational design of reduced alphabet proteins

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dc.contributor.authorDhalla, Davinder Kaur
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorWieden, Hans-Joachim
dc.contributor.supervisorRoussel, Marc R.
dc.date.accessioned2022-12-15T17:43:09Z
dc.date.available2022-12-15T17:43:09Z
dc.date.issued2022
dc.degree.levelPh.Den_US
dc.description.abstractConventional protein design approaches generally utilize a design space limited to the standard 20 amino acid alphabet (AAA), restricting the incorporation of unnatural amino acids, and associated novel functions. Reducing the standard AAA can free up codon space for the abovementioned purpose along with accelerating in silico protein design. However, previously designed reduced AAA protein variants have shown little to no activity relative to their wild-type counterparts. The overarching goal of this thesis was to identify and employ the protein design rules observed in nature for the development of a generalizable RAP design pipeline. Since dynamics are integral to maintaining protein function, data presented in this thesis investigates the structural dynamics of proteins using Molecular Dynamics simulations. This insight into the structural dynamics of proteins assisted in designing RAPs and in the characterization of a receptor targeted by a virus pathogenic to humans, offering potential pharmaceutical and astrobiology applications.en_US
dc.identifier.urihttps://hdl.handle.net/10133/6395
dc.language.isoenen_US
dc.proquest.subject0715en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : Dept. of Chemistry and Biochemistryen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectreduced alphabet proteinsen_US
dc.subjectamino acidsen_US
dc.subjectunnatural amino acidsen_US
dc.subjectamino acid alphabeten_US
dc.subjectprotein engineeringen_US
dc.subjectRAP design pipelineen_US
dc.subjectAmino acids--Researchen_US
dc.subjectAmino acids--Synthesisen_US
dc.subjectProtein engineeringen_US
dc.subjectDissertations, Academicen_US
dc.titleRational design of reduced alphabet proteinsen_US
dc.typeThesisen_US
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