Modifications of transfer RNA enhance selenoprotein biosynthesis

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dc.contributor.authorLa-Rosa Montes, Damian
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorKothe, Ute
dc.contributor.supervisorPatel, Trushar R.
dc.date.accessioned2022-07-26T17:33:15Z
dc.date.available2022-07-26T17:33:15Z
dc.date.issued2022
dc.degree.levelMastersen_US
dc.description.abstractSelenocysteine-containing proteins, selenoproteins, are generally favoured over cysteine-containing proteins in redox reactions and therefore of high interest due to their multiple bio-industrial applications. However, the unique features of Selenocysteine biosynthesis and insertion machinery limits the freedom of selenoprotein bioengineering. Whereas engineered Selenocysteine machinery alternatives have been proposed, the role of tRNA post-transcriptional modifications on selenoprotein biosynthesis remains unexplored. This thesis provides insights into the overall positive role tRNA modification on stop codon re-assignment. I have shown that by supplementing coupled transcription-translation cell-free systems with tRNA modifying enzymes, the fluorescence signal of a reporter protein is enhanced compared to protein synthesis in the absence of the modifying enzymes. I conclude that tRNA modifications, in particular N6-isopenthenyladenosine (i6A37), can potentially improve selenoprotein biosynthesis in vitro. Thus, the result of this thesis provides valuable knowledge that could contribute to future optimization of cell-free platforms for selenoprotein bioengineering.en_US
dc.description.sponsorshipUniversity of Leathbridge, Graduate Studies Office Alberta RNA Research and Training Institute NSERC Alberta Innovatesen_US
dc.identifier.urihttps://hdl.handle.net/10133/6285
dc.language.isoen_USen_US
dc.proquest.subject0487en_US
dc.proquest.subject0202en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistryen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjecttRNA modificationsen_US
dc.subjectselenoprotein biosynthesisen_US
dc.subjectselenocysteineen_US
dc.subjectTransfer RNAen_US
dc.subjectSelenoproteins--Synthesisen_US
dc.subjectBiosynthesisen_US
dc.subjectBioengineeringen_US
dc.subjectOrganoselenium compoundsen_US
dc.subjectPost-translational modificationen_US
dc.subjectProteins--Chemical modificationen_US
dc.subjectDissertations, Academicen_US
dc.titleModifications of transfer RNA enhance selenoprotein biosynthesisen_US
dc.typeThesisen_US
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