Investigation of dynamic solution interactions between NET-1 and UNC-5B by multi-wavelength analytical ultracentrifugation

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dc.contributor.authorGabir, Haben
dc.contributor.authorGupta, Monika
dc.contributor.authorMeier, Markus
dc.contributor.authorHeide, Fabian
dc.contributor.authorKoch, Manuel
dc.contributor.authorStetefeld, Joerg
dc.contributor.authorDemeler, Borries
dc.date.accessioned2024-08-28T21:35:17Z
dc.date.available2024-08-28T21:35:17Z
dc.date.issued2023
dc.descriptionAccepted author manuscript
dc.description.abstractNET-1 is a key chemotropic ligand that signals commissural axon migration and change in direction. NET-1 and its receptor UNC-5B switch axon growth cones from attraction to repulsion. The biophysical properties of the NET-1 + UNC-5B complex have been poorly characterized. Using multi-wavelength-AUC by adding a fluorophore to UNC-5B, we were able to separate the UNC-5B sedimentation from NET-1. Using both multi-wavelength- and single-wavelength AUC, we investigated NET-1 and UNC-5B hydrodynamic parameters and complex formation. The sedimentation velocity experiments show that NET-1 exists in a monomer–dimer equilibrium. A close study of the association shows that NET-1 forms a pH-sensitive dimer that interacts in an anti-parallel orientation. UNC-5B can form equimolar NET-1 + UNC-5B heterocomplexes with both monomeric and dimeric NET-1.
dc.description.peer-reviewYes
dc.identifier.citationGabir, H., Gupta, M., Meier, M., Heide, F., Koch, M., Stetefeld, J., & Demeler, B. (2023). Investigation of dynamic solution interactions between NET-1 and UNC-5B by multi-wavelength analytical ultracentrifugation. European Biophysics Journal, 52(4-5), 473-481.https://doi.org/10.1007/s00249-023-01644-
dc.identifier.urihttps://hdl.handle.net/10133/6893
dc.language.isoen
dc.publisherSpringer Link
dc.publisher.departmentDepartment of Chemistry and Biochemistry
dc.publisher.facultyArts and Science
dc.publisher.institutionUniversity of Manitoba
dc.publisher.institutionUniversity of Cologne
dc.publisher.institutionUniversity of Lethbridge
dc.publisher.institutionUniversity of Montana
dc.publisher.urlhttps://doi.org/10.1007/s00249-023-01644-
dc.subjectNetrin-1
dc.subjectUncoordinated-5
dc.subjectHetero-interaction studies between proteins
dc.subjectMulti-wavelength analytical ultracentrifugation
dc.subjectUltraScan
dc.subjectUltraScan-SOMO
dc.subjectHydrodynamic bead modeling
dc.subjectAxon guidance
dc.subject.lcshUltracentrifugation
dc.titleInvestigation of dynamic solution interactions between NET-1 and UNC-5B by multi-wavelength analytical ultracentrifugation
dc.typeBook
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