Comparative metagenomics in livestock production antimicrobial resistance

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dc.contributor.authorLee, Catrione
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorMcAllister, Tim
dc.contributor.supervisorZovoilis, Athanasios
dc.date.accessioned2024-01-25T21:27:44Z
dc.date.available2024-01-25T21:27:44Z
dc.date.issued2023
dc.degree.levelMasters
dc.description.abstractAntimicrobial resistance is a global health problem. It affects people suffering from infections and is also able to jeopardize livestock food security. With the rise of this problem, steps have been taken to reduce the use of antimicrobials in livestock production. “Raised Without” antimicrobial livestock production has been described to reduce antimicrobial resistance by removing the selective pressure which would reinforce resistance gene conservation in bacterial genomes. This thesis’ goal was to investigate the extent to which this statement is true. First, optimization of several different methods was used to detect resistance genes and associate them with mobile genetic elements to determine their transferability potential. Taking those methods and applying them to a beef cattle dataset revealed that an absence of antimicrobial use is likely not the cause of differences observed in antimicrobial resistance gene and microbiota abundance. Macrolide resistance genes are more likely to be stably conserved than tetracycline resistance genes. Expanding that analysis to include broiler chickens and swine both raised with and without antimicrobials, revealed there to be no difference between antimicrobial management practices unless at specific antimicrobial resistance gene group-levels.
dc.identifier.urihttps://hdl.handle.net/10133/6672
dc.language.isoen
dc.proquest.subject0475
dc.proquest.subject0487
dc.proquestyesYes
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry
dc.publisher.departmentDepartment of Chemistry and Biochemistry
dc.publisher.facultyArts and Science
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)
dc.subjectAntimicrobial resistance
dc.subjectAntimicrobials
dc.subjectMicrobiota
dc.subjectCattle
dc.subjectSwine
dc.subjectBroiler chickens
dc.subjectLivestock production
dc.subject.lcshAnti-infective agents
dc.subject.lcshBeef cattle
dc.subject.lcshBroilers (Chickens)
dc.subject.lcshMetagenomics
dc.subject.lcshDissertations, Academic
dc.titleComparative metagenomics in livestock production antimicrobial resistance
dc.typeThesis
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