Monitoring pathogenic Escherichia coli in Alberta beef cattle

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dc.contributor.authorConrad, Cheyenne
dc.contributor.supervisorThomas, James E.
dc.contributor.supervisorReuter, Tim
dc.date.accessioned2015-03-31T20:32:37Z
dc.date.available2015-03-31T20:32:37Z
dc.date.issued2014
dc.degree.levelMastersen_US
dc.description.abstractShiga toxin-producing Escherichia coli (STEC) are human pathogens responsible for deadly outbreaks throughout the world. Cattle are the primary reservoir for these pathogens, secreting STEC in the feces. Bovine feces is the principal source of contamination of food products, however detection of STEC in feces has several inherent limitations. The objectives of this study were to develop and refine detection methods for STEC in cattle feces. Multiplex PCRs for detection of STEC serogroups and virulence genes were designed. Serogroup detection in feces significantly improved with enrichment (10 CFU/g feces). However, isolation of target serogroups from feces remains challenging, and additional refinements to immunomagnetic separation (IMS) techniques would be an asset. Novel techniques for detection and isolation of STEC across the farm-to-fork spectrum will continue to improve food safety monitoring and reduce risks to human health by decreasing circulation of contaminated products, aiding in diagnostics, and supporting outbreak management.en_US
dc.identifier.urihttps://hdl.handle.net/10133/3651
dc.language.isoen_CAen_US
dc.proquest.subject0307en_US
dc.proquest.subject0410en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectShiga toxin-producing Escherichia coli (STEC)en_US
dc.subjectfoodborne pathogenen_US
dc.subjectfood safetyen_US
dc.titleMonitoring pathogenic Escherichia coli in Alberta beef cattleen_US
dc.typeThesisen_US
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