Douglas-fir LEAFY COTYLEDON1 (PmLEC1) is an active transcription factor during zygotic and somatic embryogenesis

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dc.contributor.authorVetrici, Mariana A.
dc.contributor.authorYevtushenko, Dmytro P.
dc.contributor.authorMisra, Santosh
dc.date.accessioned2024-09-26T21:51:28Z
dc.date.available2024-09-26T21:51:28Z
dc.date.issued2021
dc.descriptionOpen access article. Creative Commons Attribution 4.0 International license (CC BY 4.0) applies
dc.description.abstractDouglas-fir (Pseudotsuga menziesii) is one of the world's premier lumber species and somatic embryogenesis (SE) is the most promising method for rapid propagation of superior tree genotypes. The development and optimization of SE protocols in conifers is hindered by a lack of knowledge of the molecular basis of embryogenesis and limited sequence data. In Arabidopsis, the LEAFY COTYLEDON1 (AtLEC1) gene is a master regulator of embryogenesis that induces SE when expressed ectopically. We isolated the LEC1 homologue from Douglas-fir, designated as PmLEC1. PmLEC1 expression in somatic embryos and developing seeds demonstrated a unique, alternating pattern of expression with the highest levels during early stages of embryogenesis. PmLEC1 protein accumulation during seed development correlated with its transcriptional levels during early embryogenesis; however, substantial protein levels persisted until 2 weeks on germination medium. Treatment of mature, stratified seeds with 2,4-epibrassinolide, sorbitol, mannitol, or NaCl upregulated PmLEC1 expression, which may provide strategies to induce SE from mature tissues. Sequence analysis of the PmLEC1 gene revealed a 5′ UTR intron containing binding sites for transcription factors (TFs), such as ABI3, LEC2, FUS3, and AGL15, which are critical regulators of embryogenesis in angiosperms. Regulatory elements for these and other seed-specific TFs and biotic and abiotic signals were identified within the PmLEC1 locus. Most importantly, functional analysis of PmLEC1 showed that it rescued the Arabidopsis lec1-1 null mutant and, in the T2 generation, led to the development of embryo-like structures, indicating a key role of PmLEC1 in the regulation of embryogenesis.
dc.description.peer-reviewYes
dc.identifier.citationVetrici, M. A., Yevtushenko, D. P., & Misra, S. (2021). Douglas-fir LEAFY COTYLEDON1 (PmLEC1) is an active transcription factor during zygotic and somatic embryogenesis. Plant Direct, 5(7), Article e00333. https://doi.org/10.1002/pld3.333
dc.identifier.urihttps://hdl.handle.net/10133/6919
dc.language.isoen
dc.publisherWiley
dc.publisher.departmentDepartment of Biological Sciences
dc.publisher.facultyArts and Science
dc.publisher.institutionUniversity of Lethbridge
dc.publisher.institutionUniversity of Victoria
dc.publisher.urlhttps://doi.org/10.1002/pld3.333
dc.subjectDouglas-fir
dc.subjectConifer LEC1
dc.subjectSomatic embryogenesis
dc.subjectStress and hormone treatments
dc.subjectTranscription factors
dc.subject.lcshDouglas fir--Propagation
dc.titleDouglas-fir LEAFY COTYLEDON1 (PmLEC1) is an active transcription factor during zygotic and somatic embryogenesis
dc.typeArticle
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