The impact of cold and Trichostatin A treatment on the transcriptome of wheat microspores

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dc.contributor.authorJensen, Theia May
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorKovalchuk, Igor
dc.contributor.supervisorLaurie, John
dc.date.accessioned2021-10-20T17:51:41Z
dc.date.available2021-10-20T17:51:41Z
dc.date.issued2021
dc.degree.levelMastersen_US
dc.description.abstractObtaining doubled haploids plants from isolated microspores is valuable for plant breeders. Microspores generally require stress treatment to enter androgenesis and produce doubled haploids. RNA-seq analysis was performed on cultured microspores that have been subjected to a three-week cold treatment and exposure to the histone deacetylase inhibitor Trichostatin A. Results point to dramatic transcriptional reprogramming after cold pre-treatment; upregulation in transcription, cytoplasmic turn over, signal transduction through kinase cascades, and chromatin remodelling supporting a change in cell fate. Cold-stressed microspores are then able to respond to TSA treatment with transient genome-wide upregulation of several thousand genes, not seen in unstressed microspores. Pathways upregulated include mitotic cell cycle phase transition genes, transcription, and DNA recombination and repair. These pathways may explain the increase in androgenesis seen after application of TSA. Results shed light on the behavior of wheat microspores in culture and pave the way for improved androgenesis and DH production.en_US
dc.identifier.urihttps://hdl.handle.net/10133/6066
dc.language.isoen_USen_US
dc.proquest.subject0369en_US
dc.proquest.subject0479en_US
dc.proquest.subject0715en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectdoubled haploidsen_US
dc.subjectTrichostatin Aen_US
dc.subjectcold-stressed microsporesen_US
dc.subjectandrogenesisen_US
dc.subjectwheat microsporesen_US
dc.subjectwheat transcriptomeen_US
dc.subjectPlant genetics -- Researchen_US
dc.subjectHaploidy -- Researchen_US
dc.subjectAndrogenesis in plantsen_US
dc.subjectPlants -- Effect of cold onen_US
dc.subjectHistone deacetylase -- Inhibitors -- Researchen_US
dc.subjectWheat -- Genetics -- Researchen_US
dc.subjectPlant spores -- Effect of cold on -- Researchen_US
dc.subjectDissertations, Academicen_US
dc.titleThe impact of cold and Trichostatin A treatment on the transcriptome of wheat microsporesen_US
dc.typeThesisen_US
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