Distinct mitotic arrests by prairie Asteraceae plant extracts and exploration of the transcriptomic response of HT-29 cancer cells to anti-mitotic chemicals

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dc.contributor.authorAllard, Haley, Kathleen
dc.contributor.supervisorGolsteyn, Roy
dc.date.accessioned2022-01-14T17:39:42Z
dc.date.available2022-01-14T17:39:42Z
dc.date.issued2021
dc.degree.levelMastersen_US
dc.description.abstractWe investigate the mitotic activities of Canadian Asteraceae plant extracts and use two experimental approaches to study mitotic arrests. We used taxonomy and phylogeny as a basis for investigating extracts from the species Ratibida columnifera, Arnica fulgens, and Hymenopappus filifolius for anti-mitotic activity. The extracts were tested for anti-mitotic activity against cancer cells measured by rounded cell morphology and phosphorylated histone H3 assays. The extracts demonstrated distinct effects on the mitotic spindle, with different microtubule distortions and centrosome morphologies. In a second approach, we used transcriptomic analysis to compare mitotic arrests induced by sesquiterpene lactones or the cancer drug Taxol®. Transcriptomic analysis revealed differences in overall gene expression related to specific biological processes including transcripts related to microtubule regulation. Isolation of the plant extract chemicals as well as identification of protein targets may provide new chemicals suitable for studying mitosis and possible use in future precision medicines.en_US
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada, Canada Foundation for Innovation, Shell, Power Corporation of Canadaen_US
dc.identifier.urihttps://hdl.handle.net/10133/6134
dc.language.isoen_USen_US
dc.proquest.subjectCellular biology [0379]en_US
dc.proquest.subjectBotany [0309]en_US
dc.proquest.subjectBioinformatics [0715]en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biologyen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectNatural productsen_US
dc.subjectAsteraceaeen_US
dc.subjectMitosisen_US
dc.subjectTranscriptomicsen_US
dc.subjectCentrosomeen_US
dc.subjectMitotic spindleen_US
dc.subjectDissertations, Academicen_US
dc.subjectCancer cellsen_US
dc.subjectCentrosomesen_US
dc.subjectCompositaeen_US
dc.subjectPaclitaxelen_US
dc.titleDistinct mitotic arrests by prairie Asteraceae plant extracts and exploration of the transcriptomic response of HT-29 cancer cells to anti-mitotic chemicalsen_US
dc.typeThesisen_US
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