Role of conserved residues for RNA binding and catalysis in two bacterial pseudouridine synthases

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dc.contributor.authorVienneau, Hope Marie
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorKothe, Ute
dc.date.accessioned2022-01-13T17:14:24Z
dc.date.available2022-01-13T17:14:24Z
dc.date.issued2021
dc.degree.levelMastersen_US
dc.description.abstractPseudouridine synthases are RNA modification enzymes targeting various forms of RNA in the cell. RluB and RluF are pseudouridine synthases modifying adjacent uridines in E. coli ribosomal RNA. These enzymes have a highly conserved RNA-binding loop, distinct between RluB and RluF, proposed to play a role in their specificity. This thesis provides preliminary insight into the importance of the eight-residue binding loops of RluB and RluF for RNA target recognition. I have shown that altering the residues of the binding loop decreases the enzyme’s affinity for RNA and effectively abolish the enzyme’s pseudouridylation activity. I conclude that the binding loops of RluB and RluF are critical for RNA binding and for positioning the target RNA for catalysis. Better understanding the requirements for specificity and target recognition of RluB and RluF will enhance our knowledge on ribosome formation and may aid in engineering new enzymes to pseudouridylate other functional RNAs.en_US
dc.description.sponsorshipAlberta Innovates Technological Futures (AITF) National Sciences and Engineering Research Council (NSERC) University of Lethbridgeen_US
dc.identifier.urihttps://hdl.handle.net/10133/6131
dc.language.isoen_USen_US
dc.proquest.subject0487en_US
dc.proquest.subject0410en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistryen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectRNA modificationen_US
dc.subjectpseudouridineen_US
dc.subjectRNA bindingen_US
dc.subjectribosomal RNAen_US
dc.subjectRluBen_US
dc.subjectRluFen_US
dc.subjectPseudouridine--Researchen_US
dc.subjectEnzymes--Researchen_US
dc.subjectRibosomes--Researchen_US
dc.subjectRNA--Researchen_US
dc.subjectDissertations, Academicen_US
dc.titleRole of conserved residues for RNA binding and catalysis in two bacterial pseudouridine synthasesen_US
dc.typeThesisen_US
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