A molecular genetic approach to reducing the saturated fatty acid content of canola oil

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dc.contributor.authorBondaruk, Mandy
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorWeselake, Randall
dc.date.accessioned2007-04-25T19:37:12Z
dc.date.available2007-04-25T19:37:12Z
dc.date.issued2003
dc.degree.levelMasters
dc.descriptionxxi, 155 leaves : ill. ; 29 cm.en
dc.description.abstractBrassica napus is known to contain an endogenous and soluble stearoyl-acyl carrier protein (^918:0-ACP) desaturase, but does not express a palmitic (16:0)-ACP desaturase. Levels of 16:0 are low in canola oil and are associated with enhanced cholesterol biosynthesis in humans. In an attempt to further reduce the saturated fatty acid (SFA) content of canola oil, B. Napus L. cv Westar was transformed with a cDNA encoding a ^916:0-ACP desaturase from cat's claw (Doxantha unguis-cati L.). Arabidopsis thaliana was also transformed with this cDNA. Transformation of both oilseeds resulted in increased production of palmitoleic acid (^16:1) and many other effects of fatty acid composition. Overall, the SFA content did not decrease in either oilseed and investigation to why this effect occurred was examined using transgenic B. napus. Molecular genetic testing on second generation B. napus also determined the plants contained the cDNA of interest and were transcribing the cDNA.en
dc.identifier.urihttps://hdl.handle.net/10133/145
dc.language.isoen_USen
dc.publisherLethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 2003en
dc.publisher.departmentDepartment of Chemistry and Biochemistry
dc.publisher.facultyArts and Science
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en
dc.subjectSaturated fatty acidsen
dc.subjectCanola oilen
dc.subjectFatty acids -- Synthesisen
dc.subjectOilseedsen
dc.subjectOilseed plants -- Compositionen
dc.subjectMolecular geneticsen
dc.subjectDissertations, Academicen
dc.titleA molecular genetic approach to reducing the saturated fatty acid content of canola oilen
dc.typeThesisen
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