Characterization of three cysteine-rich proteins and an elongation factor from the secretome of Fusarium graminearum

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dc.contributor.authorEranthodi, Anas
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorForoud, Nora Afsaneh
dc.contributor.supervisorSchultz, Elizabeth
dc.date.accessioned2020-10-27T16:25:25Z
dc.date.available2020-10-27T16:25:25Z
dc.date.issued2020
dc.degree.levelPh.Den_US
dc.description.abstractFusarium graminearum is one of the primary causal agents of Fusarium head blight (FHB) in cereals. Most proteins in the secretome of this pathogen are uncharacterized and their role in FHB is undefined. This study was aimed at elucidating the roles of four secreted proteins in F. graminearum growth and aggressiveness; a cerato-platanin, FgCPP1, two CFEM-containing proteins, FgCFEM1 and FgCFEM2 and the elongation factor 1A (FgEF1A). Characterization of gene disruption and in locus overexpression (OX) transformants for these proteins showed that CPP1-OX produced more DON in culture and also caused higher initial infection in different wheat cultivars. CFEM1-OX accumulated less DON in culture but showed increased disease spread in a moderately susceptible wheat cultivar. FgEF1A overexpression did not lead to substantially higher FgEF1A expression; nevertheless, a reduction in fitness and aggressiveness was observed. This study provides some basic insights into the roles of these secreted proteins in F. graminearum aggressiveness.en_US
dc.identifier.urihttps://hdl.handle.net/10133/5796
dc.language.isoenen_US
dc.proquest.subject0473en_US
dc.proquest.subject0480en_US
dc.proquest.subject0307en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Department of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectFusarium graminearumen_US
dc.subjectFusarium head blighten_US
dc.subjectcysteine-rich proteinsen_US
dc.subjectplant-fungal interactionsen_US
dc.subjectdeoxynivalenolen_US
dc.subjectDONen_US
dc.subjectfungal aggressiveness factoren_US
dc.subjectgene knockout mutantsen_US
dc.subjectFusarium -- Researchen_US
dc.subjectFusarium diseases of plants -- Researchen_US
dc.subjectWheat -- Diseases and pests -- Researchen_US
dc.subjectDissertations, Academicen_US
dc.titleCharacterization of three cysteine-rich proteins and an elongation factor from the secretome of Fusarium graminearumen_US
dc.typeThesisen_US
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