A calibration disk for the correction of radial errors from chromatic abberation and rotor stretch in the Optima AUC™ analytical ultracentrifuge

Simple item page
dc.contributor.authorStoutjesdyk, Marielle
dc.contributor.authorHenrickson, Amy
dc.contributor.authorMinors, Geoff
dc.contributor.authorDemeler, Borries
dc.date.accessioned2021-07-06T19:26:11Z
dc.date.available2021-07-06T19:26:11Z
dc.date.issued2020
dc.descriptionAccepted author manuscripten_US
dc.description.abstractExperiments performed in the analytical ultracentrifuge (AUC) measure sedimentation and diffusion coefficients, as well as the partial concentration of colloidal mixtures of molecules in the solution phase. From this information, their abundance, size, molar mass, density and anisotropy can be determined. The accuracy with which these parameters can be determined depends in part on the accuracy of the radial position recordings and the boundary conditions used in the modeling of the AUC data. The AUC instrument can spin samples at speeds up to 60,000 rpm, generating forces approaching 300,000 g. Forces of this magnitude will stretch the titanium rotors used in the instrument, shifting the boundary conditions required to solve the flow equations used in the modeling of the AUC data. A second source of error is caused by the chromatic aberration resulting from imperfections in the UV–visible absorption optics. Both errors are larger than the optical resolution of currently available instrumentation. Here, we report software routines that correct these errors, aided by a new calibration disk which can be used in place of the counterbalance to provide a calibration reference for each experiment to verify proper operation of the AUC instrument. We describe laboratory methods and software routines in UltraScan that incorporate calibrations and corrections for the rotor stretch and chromatic aberration in order to support Good Manufacturing Practices for AUC data analysis.en_US
dc.description.peer-reviewYesen_US
dc.identifier.citationStoutjesdyk, M., Henrickson, A., Minors, G., & Demeler, B. (2020). A calibration disk for the correction of radial errors from chromatic aberration and rotor stretch in the Optima AUC™ analytical ultracentrifuge. European Biophysics Journal, 49, 701-709. https://doi.org/10.1007/s00249-020-01434-zen_US
dc.identifier.urihttps://hdl.handle.net/10133/5946
dc.language.isoen_USen_US
dc.publisherSpringeren_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.publisher.urlhttps://doi.org/10.1007/s00249-020-01434-zen_US
dc.subjectAnalytical ultracentrifugeen_US
dc.subjectUltraScan softwareen_US
dc.subjectInstrumentationen_US
dc.subjectGood manufacturing practicesen_US
dc.subjectReference materialsen_US
dc.subjectOptima AUC
dc.subject.lcshCentrifuges
dc.subject.lcshCalibration
dc.titleA calibration disk for the correction of radial errors from chromatic abberation and rotor stretch in the Optima AUC™ analytical ultracentrifugeen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
Demeler-calibration-disk.pdf
Size:
8.29 MB
Format:
Adobe Portable Document Format
Description:
Download
Thumbnail Image
Name:
Demeler-calibration-diskVR.pdf
Size:
7.4 MB
Format:
Adobe Portable Document Format
Description:
REQUEST a copy of the final published version
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Demeler, Borries