Transgenic assays for the analysis of DNA repair in plants

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dc.contributor.authorIlnytskyy, Yaroslav
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorKovalchuk, Igor
dc.date.accessioned2007-05-17T14:49:25Z
dc.date.available2007-05-17T14:49:25Z
dc.date.issued2005
dc.degree.levelMasters
dc.descriptionxi, 132 leaves : ill. ; 29 cm.en
dc.description.abstractIn this work we studied various aspects of DNA repair in plants, focusing mainly on point mutation repair and its interconnection with double-strand break repair. We were using transgenic point mutation and recombination substrates as a primary tool in our experiments. We have compared two transgenic homologous recombination assays (B-glucuronidase- and luciferase-based), analyzed the sensitivity of DNA repair machinery to ultraviolet radiation and assessed the involvement of AtKu80, Atm and AtXpd repair genes in point mutation repair. Ours study revealed the following: the luciferase-based recombination assay is more sensitive then B-glucuronidase-based; double-stand break repair machinery is sensitive to ultraviolet radiation, which results in increased pint mutation formation; chosen DNA repair genes might be impaired in point mutation repair, however further experimentations are needed to confirm this.en
dc.identifier.urihttps://hdl.handle.net/10133/263
dc.language.isoen_USen
dc.publisherLethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 2005en
dc.publisher.departmentDepartment of Biological Sciences
dc.publisher.facultyArts and Science
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en
dc.subjectDissertations, Academicen
dc.subjectDNA repairen
dc.subjectMutation (Biology)en
dc.subjectTransgenic plantsen
dc.titleTransgenic assays for the analysis of DNA repair in plantsen
dc.typeThesisen
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