Characterizing the interaction between PDCD4 and eIF3 with respect to translation regulation

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dc.contributor.authorSharma Khandiga, Divya
dc.contributor.supervisorThakor, Nehal
dc.contributor.supervisorRoussel, Marc R.
dc.date.accessioned2019-06-11T18:45:30Z
dc.date.available2019-06-11T18:45:30Z
dc.date.issued2017
dc.degree.levelMastersen_US
dc.description.abstractProgrammed cell death protein 4 (PDCD4) inhibits IRES-mediated translation of anti-apoptotic proteins such as XIAP. PDCD4 was shown to directly interact with the XIAP IRES element and inhibit translation initiation. Additionally, our lab reported that a eukaryotic initiation factor, eIF3 interacts with the XIAP IRES to facilitate ribosome recruitment. Interestingly, the activity of PDCD4 and eIF3 are regulated by common regulatory kinases called S6K1 and 2. Therefore, to investigate the possibility of interaction between PDCD4 and eIF3 as well as their co-regulation by S6K1 and 2, I have performed co-immunoprecipitation assays in glioblastoma and S6K double knockout MEFs. The results of in cellulo assays demonstrate RNA-independent PDCD4-eIF3 interactions. In addition, eIF3F, one of the 13 eIF3 subunits has been demonstrated to interact directly with PDCD4. This study suggests that the interaction of PDCD4 with eIF3F may have a role in regulating global and/or transcript-specific translation.en_US
dc.description.sponsorshipSGSen_US
dc.embargoYesen_US
dc.embargo.end2017-07-15
dc.embargo.start2017-01-15
dc.identifier.urihttps://hdl.handle.net/10133/5392
dc.language.isoen_USen_US
dc.proquest.subject0307en_US
dc.proquest.subject0487en_US
dc.proquest.subject0379en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Department of Chemistry and Biochemistryen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectGenetic translation -- Researchen_US
dc.subjectApoptosis -- Researchen_US
dc.subjectTumor suppressor proteins -- Researchen_US
dc.subjectDissertations, Academicen_US
dc.subjecteIF3en_US
dc.subjectPDCD4en_US
dc.subjectPDCD4-eIF3 interactionen_US
dc.subjecttranslationen_US
dc.titleCharacterizing the interaction between PDCD4 and eIF3 with respect to translation regulationen_US
dc.typeThesisen_US
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