De novo cytosine methylation in the differentiating macronucleus of the stichotrichous ciliate Stylonychia lemnae

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dc.contributor.authorJuranek, Stefan
dc.contributor.authorWieden, Hans-Joachim
dc.contributor.authorLipps, Hans J.
dc.date.accessioned2019-07-18T21:00:12Z
dc.date.available2019-07-18T21:00:12Z
dc.date.issued2003
dc.descriptionSherpa Romeo green journal. Permission to archive final published versionen_US
dc.description.abstractDramatic DNA reorganization and elimination processes occur during macronuclear differentiation in ciliates. In this study we analyzed whether cytosine methylation of specific sequences plays a functional role during DNA rearrangement. Three classes of sequences, macronuclear-destined sequences (MDSs, pCE7), members from a large family of transposon-like elements and micronuclear-specific sequences (pLJ01), differing in their structure and future destiny during nuclear differentiation, were studied in the micronucleus, the developing macronucleus and, when present, in the mature macronucleus. While the MDSs become processed to a 1.1 and 1.3 kb gene-sized macronuclear DNA molecule, the family of transposon-like elements represented by MaA81 becomes removed late in the course of polytene chromosome formation. The micronuclear-specific sequence pLJ01 is eliminated together with bulk micronuclear DNA during degradation of polytene chromosomes. No methylated cytosine could be detected in the vegetative macronucleus and no difference in methylation pattern was observed either between micronucleus and developing macronucleus in MDSs or in a micronuclear-speci®c sequence. However, a significant percentage of the cytosines contained in the transposon-like element becomes methylated de novo in the course of macronuclear differentiation. This is the first demonstration that cytosine methylation in speci®c sequences occurs during macronuclear differentiation and may provide a first step towards understanding epigenetic factors involved in DNA processingen_US
dc.description.peer-reviewYesen_US
dc.identifier.citationJuranek, S., Wieden, H.-J., & Lipps, H. J. (2003). De novo cytosine methylation in the differentiating macronucleus of the stichotrichous ciliate Stylonychia lemnae. Nucleic Acids Research, 31(5), 1387-1391. DOI: 10.1093/nar/gkg233en_US
dc.identifier.urihttps://hdl.handle.net/10133/5471
dc.language.isoen_USen_US
dc.publisherOxford University Pressen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionUniversity of Witten/Herdeckeen_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.publisher.urlhttps://doi.org/10.1093/nar/gkg233
dc.subjectCytosines
dc.subjectCytosine methylation
dc.subjectDNA reorganization
dc.subjectMacronuclear differentiation
dc.subject.lcshCytosine
dc.subject.lcshMethylation
dc.titleDe novo cytosine methylation in the differentiating macronucleus of the stichotrichous ciliate Stylonychia lemnaeen_US
dc.typeArticleen_US
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