Interaction studies of human DEAD-box helicases with viral non-coding RNA

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dc.contributor.authorNelson, Corey R.
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorPatel, Trushar R.
dc.date.accessioned2021-06-30T20:39:49Z
dc.date.available2021-06-30T20:39:49Z
dc.date.issued2021
dc.degree.levelMastersen_US
dc.description.abstractMosquito-borne illnesses are responsible for millions of deaths every year. Some of the most common diseases spread include Japanese Encephalitis, Zika, and Rift Valley Fever Virus. This thesis seeks to describe the interactions between the non-coding regions of the RNA genomes of the viruses above and DEAD-box RNA helicases DDX3X and DDX17. Microscale thermophoresis indicated that DDX3X binds the JEV 5’ RNA with a lower dissociation constant than the ZIKV 5’ but could unwind both RNAs. DDX17 binds both the intergenic non-coding and 5’ terminal RNAs from the S segment of the genome, also unwinding both. Small-angle X-ray scattering generated low-resolution 3D structures of DDX17 and the non-coding RNAs. Finally, using a baculovirus expression system has shown the potential to express full-length DDX3X but requires optimization before it is ready for downstream experimentation.en_US
dc.identifier.urihttps://hdl.handle.net/10133/5934
dc.language.isoen_USen_US
dc.proquest.subject0487en_US
dc.proquest.subject0786en_US
dc.proquest.subject0720en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistryen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectvirusen_US
dc.subjecthelicaseen_US
dc.subjectbaculovirusen_US
dc.subjectflavivirusen_US
dc.subjectinsect cell cultureen_US
dc.subjectmicroscale thermophoresisen_US
dc.subjectanalytical ultracentrifugationen_US
dc.subjectsmall angle X-ray scatteringen_US
dc.subjectFlaviviral diseases -- Researchen_US
dc.subjectBunyaviruses -- Researchen_US
dc.subjectBaculoviruses -- Geneticsen_US
dc.subjectNon-coding RNA -- Researchen_US
dc.subjectRNA viruses -- Researchen_US
dc.subjectVirus diseases -- Researchen_US
dc.subjectSmall-angle x-ray scattering -- Scientific applicationsen_US
dc.subjectUltracentrifugationen_US
dc.subjectCell cultureen_US
dc.subjectDissertations, Academicen_US
dc.titleInteraction studies of human DEAD-box helicases with viral non-coding RNAen_US
dc.typeThesisen_US
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