Applications of fecal metabolomics for the study of stress and pregnancy

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dc.contributor.authorScott, Hannah Doris
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorMetz, Gerlinde A. S.
dc.date.accessioned2021-02-08T20:38:51Z
dc.date.available2021-02-08T20:38:51Z
dc.date.issued2020
dc.degree.levelMastersen_US
dc.description.abstractThe gut microbiota plays a substantial role in determining the health and behaviour of an individual. The study of this interaction requires tools with a broad, unbiased scope that can analyse both microbial and host activity; fecal metabolomics, the analysis of small-molecule metabolites, is one such method. This thesis will explore several considerations and applications of fecal metabolomics. First, a study comparing methods of sample preparation for fecal metabolomics displays the efficacy of in silico baseline correction combined with a Bligh-Dyer extraction technique. Next, the use of fecal metabolomics and 16S rRNA gene sequencing reveal no effect of prenatal stress on the postpartum maternal gut microbiota. Finally, the clinical applications of metabolomics is presented in a study displaying distinctions between the prenatal urinary metabolomes of obesity and gestational diabetes mellitus, identifying biomarkers of risk. These studies highlight the potential of fecal metabolomics in both research and clinical settings.en_US
dc.description.sponsorshipNatural Sciences and Engineering Research Council (NSERC) of Canada Discovery Grant #05519 Diabetes, Obesity, and Nutrition Strategical Clinical Network (SCN®) Seed Granten_US
dc.identifier.urihttps://hdl.handle.net/10133/5839
dc.language.isoen_USen_US
dc.proquest.subject0317en_US
dc.proquest.subject0410en_US
dc.proquest.subject0486en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Neuroscienceen_US
dc.publisher.departmentDepartment of Neuroscienceen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectfecal metabolomicsen_US
dc.subjectgut microbiotaen_US
dc.subjectstress and pregnancyen_US
dc.subjectnuclear magnetic resonance metabolomicsen_US
dc.subject16S rRNA analysisen_US
dc.subjectMetabolites -- Researchen_US
dc.subjectFeces -- Microbiology -- Researchen_US
dc.subjectGastrointestinal system -- Microbiology -- Researchen_US
dc.subjectStress (Physiology) -- Researchen_US
dc.subjectPregnancy -- Researchen_US
dc.subjectMagnetic resonance imagingen_US
dc.subjectBiochemical markers -- Researchen_US
dc.subjectDissertations, Academicen_US
dc.titleApplications of fecal metabolomics for the study of stress and pregnancyen_US
dc.typeThesisen_US
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