Functional analysis of two baculovirus envelope proteins

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dc.contributor.authorYu, Ian-Ling
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorLung, Oliver
dc.date.accessioned2008-10-27T17:37:54Z
dc.date.available2008-10-27T17:37:54Z
dc.date.issued2008
dc.degree.levelMasters
dc.descriptionxiii, 101 leaves : ill. (some col.) ; 28 cm. --en
dc.description.abstractBudded virions of AcMNPV can enter a variety of non-host cells, a characteristic likely due to the presence of GP64, an envelope protein found on a small subset of baculoviruses. Results show that AcMNPV's tropism for vertebrate cells can be restricted - a prerequisite for using AcMNPV for targeted in vivo gene delivery - by replacing the gp64 gene with SeF from SeMNPV. Unlike the relatively well characterized GP64 protein, the significance and function of the F homolog (Ac23, a pathogenicity factor), is poorly understood. How Ac23 might contribute to the faster speed of kill was examined by comparing occlusion bodies and occlusion-derived virions (ODV) of Ac23null mutant viruses with control viruses at the ultrastructural level. The results show that Ac23null mutant produces a significantly higher percentage of ODVs with single or lower number of nucleocapsids than controls, suggesting Ac23 may play a role in multicapsid envelopment of ODVs.en
dc.identifier.urihttps://hdl.handle.net/10133/680
dc.language.isoen_USen
dc.publisherLethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 2008en
dc.publisher.departmentDepartment of Biological Sciencesen
dc.publisher.facultyArts and Scienceen
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en
dc.subjectDissertations, Academicen
dc.subjectGene expressionen
dc.subjectProteins -- Researchen
dc.subjectBaculoviruses -- Geneticsen
dc.titleFunctional analysis of two baculovirus envelope proteinsen
dc.typeThesisen
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