Molecular methods and isolates of the entomopathogenic fungus Metarhizium anisolpliae for environmentally sustainable control of grasshoppers in Canada

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dc.contributor.authorEntz, Susan Carol
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorJohnson, Dan L.
dc.date.accessioned2007-05-13T20:30:51Z
dc.date.available2007-05-13T20:30:51Z
dc.date.issued2005
dc.degree.levelMasters
dc.descriptionxv, 127 leaves : ill. (some col.) ; 28 cm.en
dc.description.abstractMetarhizium anisoplia var. acridum, a gyphomycetous fungus registered worldwide for grasshopper and locust control, is currently under consideration as a worldwide for grasshopper and locust control, is currently under consideration as a potential alternative to chemical insecticides for grasshopper control in Canada. Research in this thesis has contributed data required for the registration of biological control agents in Canada. A diagnostic PCR assay was developed for the specific detection of M. anisopliae var. acridum DNA. The assay was highly sensitive and effective for the detection of fungal DNA in infected grasshoppers. A survey of southern Alberta soils conducted in the spring of 2004 revealed the presence of Metarhizium spp. at low natural incidennce. Two indigenous isolates demonstrated pathogenicity when bioassayed against laboratory-reared and field collected grasshoppers. One of the isolates demonstrated virulence comparable to a commercial isolate. An analysis of historical weather data revealed that summer weather in the Prairie provinces should not preclude the efficacy of M. anisopliae var. acridum under local conditions.en
dc.identifier.urihttps://hdl.handle.net/10133/249
dc.language.isoen_USen
dc.publisherLethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 2005en
dc.publisher.departmentDepartment of Geography
dc.publisher.facultyArts and Science
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en
dc.subjectDissertations, Academicen
dc.subjectGrasshoppers -- Biological control -- Canadaen
dc.subjectBiological insecticidesen
dc.subjectEntomophathogenic fungien
dc.subjectGrasshoppers -- Control -- Canadaen
dc.titleMolecular methods and isolates of the entomopathogenic fungus Metarhizium anisolpliae for environmentally sustainable control of grasshoppers in Canadaen
dc.typeThesisen
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