Multi-wavelength analytical ultracentrifugation of human serum albumin complexed with porphyrin

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dc.contributor.authorJohnson, Courtney N.
dc.contributor.authorGorbet, Gary E.
dc.contributor.authorRamsower, Heidi
dc.contributor.authorUrquidi, Julio
dc.contributor.authorBrancaleon, Lorenzo
dc.contributor.authorDemeler, Borries
dc.date.accessioned2021-08-11T19:21:55Z
dc.date.available2021-08-11T19:21:55Z
dc.date.issued2018
dc.descriptionAccepted author manuscripten_US
dc.description.abstractThe new Beckman Coulter Optima AUC instrument, which features multi-wavelength detection that couples the hydro- dynamic separation of colloidal mixtures to spectral deconvolution of interacting and non-interacting solutes present in a mixture, was used to analyze the composition of human serum albumin (HSA) bound to metallo-protoporphyrin. We present new methods implemented in UltraScan that permit Optima AUC-derived multi-wavelength data to be spectrally decomposed in the same fashion as has been made possible for the Cölfen detector earlier. We demonstrate this approach by spectrally separating sedimentation velocity experimental data from mixtures of apo-HSA and HSA complexed to dif- ferent metallo-protoporphyrins. We further demonstrate how multi-wavelength AUC can accurately recover percentages of metallo-protoporphyrin-bound HSA and apo-HSA from mixtures and how multi-wavelength AUC permits the calculation of molar extinction coefficients for porphyrins bound to HSA. The presented method has broad applicability to other complex systems where mixtures of molecules with different spectral properties need to be characterized.en_US
dc.description.peer-reviewYesen_US
dc.identifier.citationJohnson, C. N., Gorbet, G. E., Ramsower, H., Urquidi, J., Brancaleon, L., & Demeler, B. (2018). Multi-wavelength analytical ultracentrifugation of human serum albumin complexed with porphyrin. European Biophysics Journal, 47(7), 789-797. https://doi.org/10.1007/s00249-018-1301-7en_US
dc.identifier.urihttps://hdl.handle.net/10133/5993
dc.language.isoen_USen_US
dc.publisherSpringeren_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionUniversity of Texas Health Science Center at San Antonioen_US
dc.publisher.institutionUniversity of Texas at San Antonioen_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.publisher.urlhttps://doi.org/10.1007/s00249-018-1301-7en_US
dc.subjectMulti-wavelength analytical ultracentrifugationen_US
dc.subjectUltraScanen_US
dc.subjectSpectral analysisen_US
dc.subjectPophyrinen_US
dc.subjectHuman serum abluminen_US
dc.subject.lcshCentrifuges
dc.titleMulti-wavelength analytical ultracentrifugation of human serum albumin complexed with porphyrinen_US
dc.typeArticleen_US
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