The translational NTPase OLA1 is an RNA binding protein

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dc.contributor.authorSemmelrock, Jessica
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorWieden, Hans-Joachim
dc.contributor.supervisorPatel, Trushar R.
dc.date.accessioned2024-01-24T18:26:43Z
dc.date.available2024-01-24T18:26:43Z
dc.date.issued2023
dc.degree.levelMasters
dc.description.abstractWith ribosome-dependent protein synthesis being essential to life, previously overlooked components of the ribosome regulome can potentially become novel therapeutic targets. Obg-like ATPase 1 (OLA1) – or YchF in bacteria – is among several universally conserved guanosine nucleotide-dependent molecular switches, called GTPases, that interact with the ribosome throughout its life cycle and provide an important fitness advantage. However, OLA1’s exact cellular role and molecular mechanism of action are poorly understood. Structural modelling reveals that OLA1 can form a tRNA-bound complex in the ribosomal A site, overall mimicking the structure of the Elongation Factor-Tu ternary complex (EF-Tu•GTP•aminoacyl tRNA) and several other translational GTPases. Using electrophoretic mobility shift assays (EMSAs) and nitrocellulose filtration, I reveal that human OLA1 can bind tRNA independent of the ribosome. Integration of these results with previous studies suggests a role for OLA1 in ribosome maintenance.
dc.identifier.urihttps://hdl.handle.net/10133/6667
dc.language.isoen
dc.proquest.subject0487
dc.proquest.subject0307
dc.proquest.subject0306
dc.proquestyesYes
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry
dc.publisher.departmentDepartment of Chemistry and Biochemistry
dc.publisher.facultyArts and Science
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)
dc.subjectTranslation
dc.subjectRibosome
dc.subjectRNA
dc.subjectYchF
dc.subjectOLA1
dc.subjectGTPase
dc.subjectBinding proteins
dc.subject.lcshRibosomes
dc.subject.lcshGuanosine triphosphates
dc.subject.lcshCarrier proteins
dc.subject.lcshDissertations, Academic
dc.titleThe translational NTPase OLA1 is an RNA binding protein
dc.typeThesis
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