Regulation of polyhydroxyalkanoic acid synthase by riboswitch in Cupriavidus necator

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dc.contributor.authorAubrey, Keith Logan
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorThakor, Nehal
dc.date.accessioned2022-05-09T17:29:27Z
dc.date.available2022-05-09T17:29:27Z
dc.date.issued2022
dc.degree.levelMastersen_US
dc.description.abstractRiboswitches provide an opportunity for inducible gene expression at the transcriptional and translational levels. The riboswitches utilized in this thesis function by creating secondary mRNA structures that sequesters the ribosome binding site (RBS), preventing translation. This secondary structure can bind to theophylline, causing the mRNA to refold, exposing the RBS and allowing translation to occur. Riboswitches are currently underutilized, tested in only a few model organisms and primarily explored through reporter genes for proof-of-concept. This thesis demonstrates the functionality of theophylline riboswitches in the industrially relevant bacteria Cupriavidus necator, and for inducible control of the industrially relevant enzyme polyhydroxyalkanoic acid synthase (phaC), both of which have previously not been demonstrated. This thesis tests the applicability of the riboswitch in industry-relevant bacteria and addresses a significant gap in the knowledge about riboswitch functionality.en_US
dc.identifier.urihttps://hdl.handle.net/10133/6170
dc.language.isoen_USen_US
dc.proquest.subject0487en_US
dc.proquest.subject0379en_US
dc.proquest.subject0307en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistryen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectriboswitchen_US
dc.subjectsynthetic biologyen_US
dc.subjectbioplasticen_US
dc.subjectRiboswitches--Researchen_US
dc.subjectSynthetic biologyen_US
dc.subjectMessenger RNAen_US
dc.subjectTheophyllineen_US
dc.subjectBiodegradable plastics--Biotechnologyen_US
dc.subjectHydrogen bacteria--Biotechnologyen_US
dc.subjectDissertations, Academicen_US
dc.titleRegulation of polyhydroxyalkanoic acid synthase by riboswitch in Cupriavidus necatoren_US
dc.typeThesisen_US
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