Stimulation of microsomal diacylglycerol acyltransferase activity from microspore-derived cell suspension cultures of oilseed rape

Simple item page
dc.contributor.authorByers, Susan D.
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorWeselake, Randall
dc.date.accessioned2007-04-24T19:51:20Z
dc.date.available2007-04-24T19:51:20Z
dc.date.issued1999
dc.degree.levelMasters
dc.descriptionxiii, 95 leaves : ill. ; 28 cm.en
dc.description.abstractSeveral factors including an unidentified endogenous substance were found to stimulate microsomal diacylglycerol acyltransferase (DGAT, EC 2.3.1.20) from a microspore-derived cell suspension culture of oilseed rape (Brassica napus L. cv Jet Neuf). Mg2+ salts were found to stimulate microsomal DGAT 14 to 23-fold. ATP and CoA were also found to stimulate the enzyme 2.4 and 12 fold respectively, although the effects were decreased in the presence of high Mg2+ concentrations. While microsomal DGAT activity was only slightly increased by the concentration of exogenous diacylglycerol in the reaction mixture it was increased substantially by the addition of exogenous phosphatidate. Other phospholipids tested were not found to have this stimulatory effect. During attempts to investigate possible covalent modification of the enzyme, the soluble fraction obtained from cell suspension homogenate was found to contain a small metastable organic molecule(s) which stimulated DGAT activity. Stimulation of microsomal DGAT by this factor was concentration dependent but not dependent on preincubation time.en
dc.identifier.urihttps://hdl.handle.net/10133/101
dc.language.isoen_USen
dc.publisherLethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 1999en
dc.publisher.departmentDepartment of Chemistry and Biochemistry
dc.publisher.facultyArts and Science
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en
dc.subjectOilseed plantsen
dc.subjectFatty acids -- Synthesisen
dc.subjectEnzymesen
dc.subjectCanola oilen
dc.subjectOilseedsen
dc.subjectAcyltransferasesen
dc.subjectPhosphorylationen
dc.subjectDissertations, Academicen
dc.titleStimulation of microsomal diacylglycerol acyltransferase activity from microspore-derived cell suspension cultures of oilseed rapeen
dc.typeThesisen
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
MQ49143.pdf
Size:
2.05 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.89 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Arts and Science, Faculty of
University of Lethbridge Theses