Characterization of the cell wall integrity MAPK pathway in Fusarium graminearum

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dc.contributor.authorGonzalez-Peña Fundora, Dianevys
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorForoud, Nora Afsaneh
dc.contributor.supervisorThakor, Nehal
dc.date.accessioned2022-10-19T15:55:44Z
dc.date.available2022-10-19T15:55:44Z
dc.date.issued2022
dc.degree.levelPh.Den_US
dc.description.abstractIn the fungal phytopathogen Fusarium graminearum, the core of the mitogen-activated protein kinase (MAPK) cascade known as the cell wall integrity (CWI) pathway is formed by Bck1 (MAPKKK), Mkk1/2 (MAPKK) and Mgv1 (MAPK). Rlm1 is the only target that has been suggested for Mgv1. The objective of this thesis was to generate and characterize F. graminearum transformants that produce a high abundance of a phosphorylated Mgv1 protein that could be used in future assays for screening its candidate targets. MGV1 in locus constitutive overexpression increased the amounts of the Mgv1 phosphorylated protein, and did not cause phenotypical changes. MKK1197E,203E in locus constitutive overexpression inhibited the expected continuous Mgv1 phosphorylation, and negatively affected mycelial growth and virulence. In addition, MKK1197E,203E_OX strains showed increased resistance to osmotic stress, and higher abundance and phosphorylation of another MAPK (Hog1), opening questions regarding the cross-talk between the CWI and the HOG MAPK pathways.en_US
dc.identifier.urihttps://hdl.handle.net/10133/6352
dc.language.isoen_USen_US
dc.proquest.subject0480en_US
dc.proquest.subject0487en_US
dc.proquest.subject0473en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistryen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectFusarium graminearumen_US
dc.subjectfusarium head blight diseaseen_US
dc.subjectwheaten_US
dc.subjectMAPKen_US
dc.subjectMgv1en_US
dc.subjectWheat--Diseases and pests--Researchen_US
dc.subjectMitogen-activated protein kinases--Researchen_US
dc.subjectFusarium diseases of plants--Researchen_US
dc.subjectMycologyen_US
dc.subjectDissertations, Academicen_US
dc.titleCharacterization of the cell wall integrity MAPK pathway in Fusarium graminearumen_US
dc.typeThesisen_US
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