Elucidating the role of human obg like ATPase1 (hOLA1) in apoptosis

dc.contributor.authorBalasingam, Nirujah
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorThakor, Nehal
dc.contributor.supervisorWieden, Hans-Joachim
dc.date.accessioned2018-07-18T22:06:42Z
dc.date.available2018-07-18T22:06:42Z
dc.date.issued2017
dc.degree.levelMastersen_US
dc.description.abstracthOLA1 is a purine nucleotide binding protein that belongs to the P-loop GTPase family. hOLA1 suppresses protein synthesis by limiting ternary complex formation thus promoting the integrated stress response (ISR). ISR determines cell fate by activating or inhibiting pro- and anti-apoptotic proteins depending on the type of the stress condition. ISR facilitates cell survival in response to mild stress and promotes apoptosis in response to chronic stress. Depletion of hOLA1 leads to increased cell survival and diminished ISR. This study investigates the role of hOLA1 in apoptosis. My hypothesis was that diminished ISR in hOLA1-depleted cells leads to upregulation of anti-apoptotic proteins thus inhibiting apoptosis. Indeed, this study shows that hOLA1 depletion upregulates anti-apoptotic proteins such as cIAP1, cIAP2, and Bcl-xL, thus inhibiting apoptosis. The inhibition of apoptosis was studied by assessing caspase activation, cleaved poly (ADP-ribose) polymerase (PARP) level, and measuring apoptosis by propidium iodide (PI) staining and flow cytometry.en_US
dc.description.sponsorshipNSERC ARRTI Innovation Canadaen_US
dc.embargoNoen_US
dc.identifier.urihttps://hdl.handle.net/10133/5173
dc.language.isoen_USen_US
dc.proquest.subject0379en_US
dc.proquest.subject0307en_US
dc.proquest.subject0487en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : Universtiy of Lethbridge, Department of Chemistry and Biochemistryen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectAdenosine triphosphataseen_US
dc.subjectApoptosisen_US
dc.subjectPurine nucleotidesen_US
dc.subjectProtein bindingen_US
dc.subjectBreast -- Canceren_US
dc.subjectGenetic translationen_US
dc.subjectbreast canceren_US
dc.subjectcanceren_US
dc.subjecthOLA1en_US
dc.subjecttranslationen_US
dc.titleElucidating the role of human obg like ATPase1 (hOLA1) in apoptosisen_US
dc.typeThesisen_US
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