The effect of cannabis extracts on the expression of fibrosis-related genes

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dc.contributor.authorPryimak, Nazar
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorKovalchuk, Igor
dc.contributor.supervisorKovalchuk, Olga
dc.date.accessioned2021-11-23T18:32:34Z
dc.date.available2021-11-23T18:32:34Z
dc.date.issued2021
dc.degree.levelMastersen_US
dc.description.abstractFibrosis is a condition characterized by thickening and/or scarring of the respective tissue. Up till now, we can only try to prevent this condition, and unfortunately, it is almost impossible to reverse it. Therefore, new therapeutic interventions are urgently needed. It has been shown in the literature that cannabinoids, especially cannabidiol and Δ9-tetrahydrocannabinol and Cannabis sativa extracts have potent anti-inflammatory properties and thus may also exhibit anti-fibrotic effects. Our lab has developed several cannabis extracts that demonstrated antiinflammatory properties. Here, we hypothesized that these high CBD extracts may also have anti-fibrotic action. In this research, we utilized commercially available 3D-tissues to induce fibrosis. Next, we studied the effects of cannabis extracts on the expression of genes involved in the major fibrosis-related pathways using RNA sequencing and qRT-PCR. We discovered that high CBD cannabis extracts downregulate the expression of several key fibrotic genes indicating their anti-fibrotic potential at the transcriptional level.en_US
dc.identifier.urihttps://hdl.handle.net/10133/6095
dc.language.isoen_USen_US
dc.proquest.subject0571en_US
dc.proquest.subject0307en_US
dc.proquest.subject0715en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biologyen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectfibrosisen_US
dc.subjectcannabinoidsen_US
dc.subjectanti-fibrotic effectsen_US
dc.subjectCBD extractsen_US
dc.subjectfibrotic gene expressionen_US
dc.subjectFibrosis -- Researchen_US
dc.subjectFibrosis -- Treatment -- Researchen_US
dc.subjectCannabinoids -- Therapeutic use -- Researchen_US
dc.subjectCannabis -- Therapeutic use -- Researchen_US
dc.subjectGenetic transcription -- Regulationen_US
dc.subjectGene expressionen_US
dc.subjectGenetic regulationen_US
dc.subjectMateria medica, Vegetableen_US
dc.subjectMedicinal plants -- Researchen_US
dc.subjectDissertations, Academicen_US
dc.titleThe effect of cannabis extracts on the expression of fibrosis-related genesen_US
dc.typeThesisen_US
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