Characterization of cell-penetrating peptide complexation and interaction with plant cells

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dc.contributor.authorPepper, Jordan Tan
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorEudes, François
dc.contributor.supervisorKovalchuk, Igor
dc.date.accessioned2016-07-04T17:00:44Z
dc.date.available2016-07-04T17:00:44Z
dc.date.issued2015
dc.degree.levelMastersen_US
dc.description.abstractCell penetrating peptides (CPPs) are short 8-30 amino acid polypeptides that are able to transit across the semi-permeable plasma membrane of living cells, and deliver bound cargoes to the cytoplasm and other subcellular locations (e.g. nucleus, plastid organelles etc). The use of CPPs in plant cells and tissues remains a small and little researched field compared with the greater body of animal based research imperatives. The present research describes firstly, the measured interaction of the chosen model CPP Tat¬2 with the cell wall of microspores (i.e. microspore exine), secondly the reduction of the size and increase in regularity (measured as polydispersity) of Tat2 nucleic acid polyplexes and the unique empirical derivation of pseudo-kinetic parameters of nucleic acid CPP aggregation. Additionally several attempts to apply the findings from this research in plant cells and tissues are presented and discussed throughout.en_US
dc.description.sponsorshipAgriculture and Agri-food Canada/Agriculture et Agroalimentaire Canada (AAFC-AAC)en_US
dc.embargoYesen_US
dc.embargo.end2016-06-30
dc.embargo.start2016-01-01
dc.identifier.urihttps://hdl.handle.net/10133/4532
dc.language.isoen_CAen_US
dc.proquest.subject0307en_US
dc.proquest.subject0379en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta : University of Lethbridge, Dept. of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectCell-penetrating peptidesen_US
dc.subjectZeta-potentialen_US
dc.subjectDynamic light scatteringen_US
dc.subjectTriticaleen_US
dc.subjectsiRNAen_US
dc.subjectdsRNAen_US
dc.subjectMicrosporeen_US
dc.titleCharacterization of cell-penetrating peptide complexation and interaction with plant cellsen_US
dc.typeThesisen_US
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