Development of a molecular-based risk assessment assay for human pathogenic Campylobacter jejuni

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dc.contributor.authorBuchanan, Cody J.
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorThomas, James E.
dc.contributor.supervisorTaboada, Eduardo N.
dc.date.accessioned2019-05-22T21:23:37Z
dc.date.available2019-05-22T21:23:37Z
dc.date.issued2018
dc.degree.levelMastersen_US
dc.description.abstractDespite the fact that C. jejuni is a leading cause of bacterial enteritis in Canada, there is no diagnostic assay available to identify high-risk strains. This represents a significant challenge towards the prevention and control of campylobacteriosis. Additionally, molecular epidemiological studies have shown that not all C. jejuni strains or lineages appear to pose an equal risk to human health, and the factors underlying this subtype-dependent pathogenesis are not understood. A Genome-Wide Association Study was conducted to identify genetic markers associated with C. jejuni strains linked to human illness. These markers were implemented in a molecular-based risk assessment assay, and used to screen isolates collected as part of a national microbial survey of Canadian poultry products. This study suggests that strains with a diverse metabolic toolkit may pose an elevated risk by virtue of their ability to overcome ecological barriers that might otherwise mitigate exposure to humans.en_US
dc.embargoNoen_US
dc.identifier.urihttps://hdl.handle.net/10133/5365
dc.language.isoen_USen_US
dc.proquest.subject0306en_US
dc.proquest.subject0410en_US
dc.proquest.subject0573en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : Universtiy of Lethbridge, Department of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectCampylobacter jejuni -- Researchen_US
dc.subjectCampylobacter infections -- Epidemiologyen_US
dc.subjectBacterial geneticsen_US
dc.subjectBacterial genomesen_US
dc.subjectPathogenic bacteriaen_US
dc.subjectFoodborne diseases -- Microbiologyen_US
dc.subjectDiagnostic bacteriologyen_US
dc.subjectDissertations, Academicen_US
dc.subjectdiagnostic assayen_US
dc.subjectgastrointestinal illness causing pathogensen_US
dc.subjecthigh-risk strain identificationen_US
dc.subjecthuman campylobacteriosisen_US
dc.subjectwhole genome typingen_US
dc.titleDevelopment of a molecular-based risk assessment assay for human pathogenic Campylobacter jejunien_US
dc.typeThesisen_US
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