Current and future experimental strategies for structural analysis of trichothecene mycotoxins-a prospectus

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dc.contributor.authorShank, Roxanne A.
dc.contributor.authorForoud, Nora Afsaneh
dc.contributor.authorHazendonk, Paul
dc.contributor.authorEudes, François
dc.contributor.authorBlackwell, Barbara A.
dc.date.accessioned2016-07-05T20:15:11Z
dc.date.available2016-07-05T20:15:11Z
dc.date.issued2011
dc.descriptionOpen accessen_US
dc.description.abstractFungal toxins, such as those produced by members of the order Hypocreales, have widespread effects on cereal crops, resulting in yield losses and the potential for severe disease and mortality in humans and livestock. Among the most toxic are the trichothecenes. Trichothecenes have various detrimental effects on eukaryotic cells including an interference with protein production and the disruption of nucleic acid synthesis. However, these toxins can have a wide range of toxicity depending on the system. Major differences in the phytotoxicity and cytotoxicity of these mycotoxins are observed for individual members of the class, and variations in toxicity are observed among different species for each individual compound. Furthermore, while diverse toxicological effects are observed throughout the whole cellular system upon trichothecene exposure, the mechanism of toxicity is not well understood. In order to comprehend how these toxins interact with the cell, we must first have an advanced understanding of their structure and dynamics. The structural analysis of trichothecenes was a subject of major interest in the 1980s, and primarily focused on crystallographic and solution-state Nuclear Magnetic Resonance (NMR) spectroscopic studies. Recent advances in structural determination through solution- and solid-state NMR, as well as computation based molecular modeling is leading to a resurgent interest in the structure of these and other mycotoxins, with the focus shifting in the direction of structural dynamics. The purpose of this work is to first provide a brief overview of the structural data available on trichothecenes and a characterization of the methods commonly employed to obtain such information. A summary of the current understanding of the relationship between structure and known function of these compounds is also presented. Finally, a prospectus on the application of new emerging structural methods on these and other related systems is discussed.en_US
dc.description.peer-reviewYesen_US
dc.identifier.citationShank, R.A., Foroud, N.A., Hazendonk, P., Eudes, F., & Blackwell, B.A. (2011). Current and future experimental strategies for structural analysis of trichothecene mycotoxins-a prospectus. Toxins, 3, 1518-1553. doi:10.3390/toxins3121518en_US
dc.identifier.urihttps://hdl.handle.net/10133/4553
dc.language.isoen_CAen_US
dc.publisherM D P I A Gen_US
dc.publisher.departmentDepartment of Chemistry & Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionAgriculture and Agri-Food Canadaen_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.subjectTrichotheceneen_US
dc.subjectNMRen_US
dc.subjectSolid-state NMRen_US
dc.subjectCrystallographyen_US
dc.subjectNMR crystallographyen_US
dc.subjectNatural producten_US
dc.subjectMycotoxinen_US
dc.subjectStructureen_US
dc.subjectStructure-functionen_US
dc.subjectMolecular modelingen_US
dc.subjectMolecular dynamicsen_US
dc.subjectAntibioticen_US
dc.subjectRibosomeen_US
dc.titleCurrent and future experimental strategies for structural analysis of trichothecene mycotoxins-a prospectusen_US
dc.typeArticleen_US
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