Metabolomics of stress : assessing bio-fluids in rats and humans using proton nuclear magnetic resonance (1H NMR) spectroscopy

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dc.contributor.authorPaxman, Eric James
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorMetz, Gerlinde A. S.
dc.date.accessioned2018-03-12T20:20:04Z
dc.date.available2018-03-12T20:20:04Z
dc.date.issued2017
dc.degree.levelMastersen_US
dc.description.abstractStress in utero causes epigenetic changes that manifest in offspring physiology and behaviour, becoming risk factors for complex adult diseases. Here we used 1H NMR spectroscopy to analyze two novel cohorts; first, we assessed urine metabolomes of adolescents in utero during the largest natural disaster in Canadian history; second, we investigated blood plasma metabolomes of offspring that were transgenerationally or multigenerationally-stressed in utero. Metabolomic profiles in offspring of high PNMS mothers were significantly altered. Higher PNMS generated significant alterations in metabolic pathways involving energy metabolism and protein biosynthesis in both rats and humans, particularly in BCAA synthesis, TCA cycle, muscle performance, and immunoregulation. Dysregulation of energy and protein metabolism suggests an increased risk of metabolic diseases, such as insulin resistance, diabetes, and obesity. The present findings provide a novel approach to PNMS outcomes and HPA-axis dysregulation by taking a metabolomics perspective and utilizing novel stressors in animal and human models.en_US
dc.description.sponsorshipThis research was funded by CIHR (MOP-111177), NSERC #5519, CIHR #363195 and University of Lethbridge. EP was supported by a CIHR CGS-M award.en_US
dc.embargoNoen_US
dc.identifier.urihttps://hdl.handle.net/10133/5037
dc.language.isoen_USen_US
dc.proquest.subject0487en_US
dc.proquest.subject0306en_US
dc.proquest.subject0317en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : Universtiy of Lethbridge, Department of Neuroscienceen_US
dc.publisher.departmentDepartment of Neuroscienceen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectepigentic changesen_US
dc.subjectmetabolomicsen_US
dc.subjectprenatal maternal stressen_US
dc.subjectstress in uteroen_US
dc.subjectPrenatal influencesen_US
dc.subjectStress (Physiology)en_US
dc.subjectMetabolitesen_US
dc.titleMetabolomics of stress : assessing bio-fluids in rats and humans using proton nuclear magnetic resonance (1H NMR) spectroscopyen_US
dc.typeThesisen_US
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