Characterization of small nucleolar RNAs in the protist organism Euglena gracilis

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dc.contributor.authorMoore, Ashley N.
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorRussell, Anthony G.
dc.date.accessioned2015-07-16T21:55:22Z
dc.date.available2015-07-16T21:55:22Z
dc.date.issued2015
dc.degree.levelPh.Den_US
dc.description.abstractNon-coding RNAs (ncRNAs) have diverse cellular roles in all three domains of life. One class of ncRNAs termed small nucleolar RNAs (snoRNAs) play a role in RNA modification and processing in archaea and eukaryotic organisms. ncRNAs (including snoRNAs) remain largely unexplored in a group of unicellular eukaryotes known as protists. The focus of this study was to characterize snoRNAs in the protist organism Euglena gracilis in terms of their genomic arrangement, expression and evolution using experimental and computational methods. Numerous novel snoRNAs were characterized, many of which reside in tandemly repeated clusters that can be transcribed polycistronically. Some snoRNA gene clusters are surprisingly large, possibly the largest characterized to date. A mechanism of snoRNA evolution to support the unusually large number of snoRNAs and clustered modification sites in E. gracilis was characterized. These findings exemplify snoRNA diversity and highlight the importance of ncRNA characterization in a broad range of organisms.en_US
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada (NSERC) (Discovery Grant and Alexander Graham Bell Canada Graduate Scholarships).en_US
dc.embargoNoen_US
dc.identifier.urihttps://hdl.handle.net/10133/3715
dc.language.isoen_CAen_US
dc.proquest.subject0369en_US
dc.proquest.subject0307en_US
dc.proquest.subject0715en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectSmall nucleolar RNAen_US
dc.subjectEvolutionen_US
dc.subjectEuglena gracilisen_US
dc.subjectProtisten_US
dc.subjectNon-coding RNAen_US
dc.subjectSmall RNA libraryen_US
dc.subjectGene organizationen_US
dc.subjectGene expressionen_US
dc.titleCharacterization of small nucleolar RNAs in the protist organism Euglena gracilisen_US
dc.typeThesisen_US
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