Detection and serotyping of foot-and-mouth disease virus with laboratory and in silico methods

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dc.contributor.authorOrtega Polo, Rodrigo
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorLung, Oliver
dc.contributor.supervisorGolsteyn, Roy
dc.date.accessioned2018-12-10T22:53:03Z
dc.date.available2018-12-10T22:53:03Z
dc.date.issued2018
dc.degree.levelMastersen_US
dc.description.abstractFoot-and-mouth disease virus (FMDV) is a highly contagious animal pathogen and it has a variable genome and high antigenic variation. There are seven known serotypes of this virus: A, O, C, Asia1, SAT1, SAT2, and SAT3. The rapid detection and serotype characterization of the virus is instrumental for the prompt response by animal health authorities. This thesis presents the design and development of the first electronic microarray assay for the simultaneous detection and subtyping of FMDV. The assay was evaluated in silico and it was tested with 19 synthetic DNA constructs representing all 7 serotypes, followed by the testing with 23 viral RNA samples representing all 7 serotypes. Also, various in silico methods were compared for the classification of FMDV sequences using complete genomes and next generation sequencing (NGS) data. Finally, highly specific and highly sensitive single nucleotide variant signatures that distinguish the seven FMDV serotypes were discovered.en_US
dc.description.sponsorshipChemical, Biological, Radiological-Nuclear, and Explosives Research and Technology Initiative (CRTI) Project 09-403TAen_US
dc.embargoNoen_US
dc.identifier.urihttps://hdl.handle.net/10133/5257
dc.language.isoen_USen_US
dc.proquest.subject0720en_US
dc.proquest.subject0715en_US
dc.proquest.subject0307en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : Universtiy of Lethbridge, Department of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectFoot-and-mouth disease virus -- Identificationen_US
dc.subjectFoot-and-mouth disease -- Epidemiologyen_US
dc.subjectMolecular virologyen_US
dc.subjectVeterinary diagnostic virologyen_US
dc.subjectDNA microarraysen_US
dc.subjectCommunicable diseases in animals -- Diagnosisen_US
dc.subjectelectronic microarray assayen_US
dc.subjectFMDV detectionen_US
dc.subjectFMDV subtypingen_US
dc.subjectveterinary diagnosticsen_US
dc.subjectDissertations, Academicen_US
dc.titleDetection and serotyping of foot-and-mouth disease virus with laboratory and in silico methodsen_US
dc.typeThesisen_US
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