Non-therapeutic administration of a model antimicrobial growth promoter modulates intestinal immune responses

dc.contributor.authorCosta, Estela
dc.contributor.authorUwiera, Richard R. E.
dc.contributor.authorKastelic, John P.
dc.contributor.authorSelinger, L. Brent
dc.contributor.authorInglis, G. Douglas
dc.descriptionSherpa Romeo green journal: open accessen_US
dc.description.abstractBackground: The development of efficacious alternatives to antimicrobial growth promoters (AGP) in livestock production is an urgent issue, but is hampered by a lack of knowledge regarding the mode of action of AGP. The belief that AGP modulate the intestinal microbiota has become prominent in the literature; however, there is a lack of experimental evidence to support this hypothesis. Using a chlortetracycline-murine-Citrobacter rodentium model, the ability of AGP to modulate the intestinal immune system in mammals was investigated. Results: C. rodentium was transformed with the tetracycline resistance gene, tetO, and continuous oral administration of a non-therapeutic dose of chlortetracycline to mice did not affect densities of C. rodentium CFU in feces throughout the experiment or associated with mucosal surfaces in the colon (i.e. at peak and late infection). However, chlortetracycline regulated transcription levels of Th1 and Th17 inflammatory cytokines in a temporal manner in C. rodentium-inoculated mice, and ameliorated weight loss associated with infection. In mice inoculated with C. rodentium, those that received chlortetracycline had less pathologic changes in the distal colon than mice not administered CTC (i.e. relative to untreated mice). Furthermore, chlortetracycline administration at a non-therapeutic dose did not impart either prominent or consistent effects on the colonic microbiota. Conclusion: Data support the hypothesis that AGP function by modulating the intestinal immune system in mammals. This finding may facilitate the development of biorationale-based and efficacious alternatives to AGP.en_US
dc.identifier.citationCosta, E., Uwiera, R.R.E., Kastelic, J.P., Selinger, L.B., & Inglis, G.D. (2011). Non-therapeutic adminstration of a model antimicrobial growth promoter modulates intestinal immune responses. Gut Pathogens, 3(14).doi:10.1186/1757-4749-3-14:en_US
dc.publisherBioMed Centralen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionLethbridge Research Centreen_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.publisher.institutionUniversity of Albertaen_US
dc.subjectAntimicrobial growth promotersen_US
dc.subjectCitrobacter rodentiumen_US
dc.subjectImmunomodulation hypothesisen_US
dc.titleNon-therapeutic administration of a model antimicrobial growth promoter modulates intestinal immune responsesen_US
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