Transcriptomic and metatranscriptomic approaches to characterizing genes coding for fiber digestion within the rumen ecosystem

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dc.contributor.authorWang, Pan
dc.contributor.supervisorSelinger, L. Brent
dc.contributor.supervisorMcAllister, Tim A.
dc.date.accessioned2014-07-02T20:56:22Z
dc.date.available2014-07-02T20:56:22Z
dc.date.issued2013
dc.degree.grantorPhD
dc.degree.levelPh.Den_US
dc.descriptionxiv leaves : ill. (some col.) ; 29 cmen_US
dc.description.abstractThe rumen microbiome constitutes a unique genetic resource of plant fiber degrading microbial enzymes that could be used for agricultural and industrial purposes. Anaeromyces mucronatus is a poorly characterized anaerobic lignocellulolytic fungus in the rumen. This thesis aimed at better understanding A. mucronatus YE505 and the particle associated rumen microbiota based on transcriptomic and metatranscriptomic approaches. High quality RNA was isolated from the fiber-associated rumen sample based on an improved RNA extraction method. A transcriptomic study was performed to investigate the expression of the fiber degrading system of A. mucronatus YE505, and the functional diversity of the fiber-associated eukaryotes from the rumen of muskoxen (Ovibos moschatus) was explored by a metatranscriptomic study. Much carbohydrate degradation related protein modules were detected. This study established effective approaches to characterizing the functional contents of rumen eukaryotic microbiome as well as rumen fungi, and identified several candidate genes that merit further investigation.en_US
dc.identifier.urihttps://hdl.handle.net/10133/3459
dc.language.isoen_CAen_US
dc.proquestyesNoen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectRumen -- Microbiology -- Researchen_US
dc.subjectRumen fermentation -- Researchen_US
dc.subjectRuminants -- Digestive organsen_US
dc.subjectAnaerobic fungi -- Researchen_US
dc.subjectNucleotide sequenceen_US
dc.subjectRNA -- Analysisen_US
dc.subjectMicrobial metabolismen_US
dc.subjectDissertations, Academicen_US
dc.titleTranscriptomic and metatranscriptomic approaches to characterizing genes coding for fiber digestion within the rumen ecosystemen_US
dc.typeThesisen_US
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