Stochastic modeling of the torpedo mechanism of eukaryotic transcription termination

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dc.contributor.authorMurphy, Reba-Jean
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorRoussel, Marc R.
dc.date.accessioned2017-09-08T22:06:27Z
dc.date.available2017-09-08T22:06:27Z
dc.date.issued2017
dc.degree.levelMastersen_US
dc.description.abstractThe torpedo mechanism is a type of eukaryotic transcription termination completed by RNAPII and consists of a chase-down of RNAPII by the exonuclease Xrn2 on the 3' flanking region of the RNA transcript. A biochemical model of the mechanism is detailed. By applying Gillespie simulations and using a one-dimensional biased random walk, the effects of the relative speeds of RNAPII to Xrn2 and the size of the intergenic region on termination via the torpedo mechanism are explored. The success rates of termination in terms of the likelihood of downstream gene interference, the distribution of successful termination times, and the distribution of nucleotides synthesized by RNAPII in the termination region are discussed.en_US
dc.description.sponsorshipUniversity of Lethbridgeen_US
dc.embargoNoen_US
dc.identifier.urihttps://hdl.handle.net/10133/4906
dc.language.isoen_USen_US
dc.proquest.subject0487en_US
dc.proquest.subject0405en_US
dc.proquest.subject0494en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta : University of Lethbridge, Dept. of Chemistry and Biochemistryen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectbiochemical modelsen_US
dc.subjectGillespie algorithmsen_US
dc.subjectrandom walksen_US
dc.subjecttorpedo termination mechanismen_US
dc.subjecttranscription terminationen_US
dc.titleStochastic modeling of the torpedo mechanism of eukaryotic transcription terminationen_US
dc.typeThesisen_US
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