Hide and seek: investigation of biodiversity and distribution of cryptic duckweed species in Alberta

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dc.contributor.authorSenevirathna, Kanishka Madushanka
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorLaird, Robert A.
dc.contributor.supervisorBurg, Theresa M.
dc.date.accessioned2021-02-27T17:44:46Z
dc.date.available2021-02-27T17:44:46Z
dc.date.issued2021
dc.degree.levelMastersen_US
dc.description.abstractThis thesis focused on the development of molecular tools to characterize the biogeographical distribution of two cryptic Lemna species suspected to be present in Alberta, Lemna turionifera and L. minor. I developed eight speciesā€specific primers for five morphologically similar Lemna species. Our preliminary data show L. minor is present in multiple wetlands in southern Alberta. Subsequently, I used genotyping-by-sequencing technology to study the population diversity of these cryptic Lemna species. Based on analyses of 103 samples at 16,007 single nucleotide polymorphism loci, I provide strong support for genetic diversity existing between L. minor and L. turionifera growing in Alberta. I found significant population differentiation in L. minor but no genetically distinct populations within L. turionifera. In summary, DNA barcoding allowed us to exclusively identify unknown specimens to species level. This study provides an example of using next generation sequencing methods to identify significant fine-scale population genetic variations.en_US
dc.description.sponsorshipThis research was supported by an Alberta Conservation Association Research Grant (grant no. 015-00-90-281 [RAL and TMB]) and two Natural Sciences and Engineering Research Council of Canada Discovery Grants (grant no. RGPIN-2015-05486 [RAL] and 2019-05068[TMB]).en_US
dc.identifier.urihttps://hdl.handle.net/10133/5841
dc.language.isoen_USen_US
dc.proquest.subject0715en_US
dc.proquest.subject0369en_US
dc.proquest.subject0307en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectPopulation geneticsen_US
dc.subjectNext-generation sequencingen_US
dc.subjectDNA barcodingen_US
dc.subjectLemna turioniferaen_US
dc.subjectLemna minoren_US
dc.subjectcryptic Lemna speciesen_US
dc.subjectPlant population genetics -- Albertaen_US
dc.subjectLemna -- Alberta -- Classification -- Molecular aspectsen_US
dc.subjectLemna minor -- Alberta -- Classification -- Molecular aspectsen_US
dc.subjectLemna -- Alberta -- Molecular geneticsen_US
dc.subjectGenomics -- Data processingen_US
dc.subjectDNA fingerprinting of plantsen_US
dc.subjectPlant genome mappingen_US
dc.subjectNucleotide sequence -- Data processingen_US
dc.subjectDissertations, Academicen_US
dc.titleHide and seek: investigation of biodiversity and distribution of cryptic duckweed species in Albertaen_US
dc.typeThesisen_US
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