Influence of pathogenic bacterial determinants on genome stability of exposed intestinal cells and of distal liver and spleen cells

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dc.contributor.authorWalz, Paul S
dc.contributor.supervisorKovalchuk, Igor
dc.contributor.supervisorKovalchuk, Olga
dc.date.accessioned2014-05-01T21:51:31Z
dc.date.available2014-05-01T21:51:31Z
dc.date.issued2011
dc.degree.levelMastersen_US
dc.degree.levelMasters
dc.descriptionxiv, 132 leaves : ill. (chiefly col.) ; 29 cmen_US
dc.description.abstractMost bacterial infections can be correlated to contamination of consumables such as food and water. Upon contamination, boil water advisories have been ordered to ensure water is safe to consume, despite the evidence that heat-killed bacteria can induce genomic instability of exposed (intestine) and distal cells (liver and spleen). We hypothesize that exposure to components of heat-killed Escherichia coli O157:H7 will induce genomic instability within animal cells directly and indirectly exposed to these determinants. Mice were exposed to various components of dead bacteria such as DNA, RNA, protein or LPS as well as to whole heat-killed bacteria via drinking water. Here, we report that exposure to whole heat-killed bacteria and LPS resulted in significant alterations in the steady state RNA levels and in the levels of proteins involved in proliferation, DNA repair and DNA methylation. Exposure to whole heat-killed bacteria and their LPS components also leads to increased levels of DNA damage.en_US
dc.identifier.urihttps://hdl.handle.net/10133/3405
dc.language.isoen_CAen_US
dc.proquestyesNoen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biology, c2011en_US
dc.publisher.departmentDepartment of Biologyen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectPathogenic bacteria -- Inactivation -- Researchen_US
dc.subjectEscherichia coli O157:H7 -- Researchen_US
dc.subjectMicrobial carcinogenesis -- Researchen_US
dc.subjectDNA damage -- Researchen_US
dc.subjectIntestines -- Infections -- Pathogenesisen_US
dc.subjectLiver cellsen_US
dc.subjectSpleen -- Pathogenesis -- Researchen_US
dc.subjectEndotoxins -- Researchen_US
dc.subjectMice as laboratory animalsen_US
dc.subjectDissertations, Academicen_US
dc.titleInfluence of pathogenic bacterial determinants on genome stability of exposed intestinal cells and of distal liver and spleen cellsen_US
dc.typeThesisen_US
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