The elucidation of stress memory inheritance in Brassica rapa plants

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dc.contributor.authorBilichak, Andriy
dc.contributor.authorIlnytskyy, Yaroslav
dc.contributor.authorWoycicki, Rafal
dc.contributor.authorKepeshchuk, Nina
dc.contributor.authorFogen, Dawson
dc.contributor.authorKovalchuk, Igor
dc.date.accessioned2016-10-31T21:34:49Z
dc.date.available2016-10-31T21:34:49Z
dc.date.issued2015
dc.descriptionSherpa Romeo green journal: open accessen_US
dc.description.abstractPlants are able to maintain the memory of stress exposure throughout their ontogenesis and faithfully propagate it into the next generation. Recent evidence argues for the epigenetic nature of this phenomenon. Small RNAs (smRNAs) are one of the vital epigenetic factors because they can both affect gene expression at the place of their generation and maintain non-cell-autonomous gene regulation. Here, we have made an attempt to decipher the contribution of smRNAs to the heat-shock-induced transgenerational inheritance in Brassica rapa plants using sequencing technology. To do this, we have generated comprehensive profiles of a transcriptome and a small RNAome (smRNAome) from somatic and reproductive tissues of stressed plants and their untreated progeny. We have demonstrated that the highest tissue-specific alterations in the transcriptome and smRNAome profile are detected in tissues that were not directly exposed to stress, namely, in the endosperm and pollen. Importantly, we have revealed that the progeny of stressed plants exhibit the highest fluctuations at the smRNAome level but not at the transcriptome level. Additionally,we have uncovered the existence of heat-inducible and transgenerationally transmitted tRNA-derived small RNA fragments in plants. Finally,we suggest that miR168 and braAGO1 are involved in the stress-induced transgenerational inheritance in plantsen_US
dc.description.peer-reviewYesen_US
dc.identifier.citationBilichak, A., Ilnytskyy, Y., Woycicki, R., Kepeshchuk, N., Fogen, D., & Kovalchuk, I. (2015). The elucidation of stress memory inheritance in Brassica rapa plants. Frontiers in Plant Science, 6(5). doi:10.3389/fpls.2015.00005en_US
dc.identifier.urihttps://hdl.handle.net/10133/4639
dc.language.isoen_CAen_US
dc.publisherFrontiers Mediaen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionLethbridge Research Centreen_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.subjectEpigeneticsen_US
dc.subjectTranscriptomeen_US
dc.subjectSmall RNAsen_US
dc.subjectB. rapaen_US
dc.subjectTransgenerational inheritanceen_US
dc.titleThe elucidation of stress memory inheritance in Brassica rapa plantsen_US
dc.typeArticleen_US
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