Spectral and hydrodynamic analysis of West Nile virus RNA–protein interactions by multiwavelength sedimentation velocity in the analytical ultracentrifuge

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dc.contributor.authorZhang, Jin
dc.contributor.authorPearson, Joseph Z.
dc.contributor.authorGorbet, Gary E.
dc.contributor.authorCölfen, Helmut
dc.contributor.authorGermann, Markus W.
dc.contributor.authorBrinton, Margo A.
dc.contributor.authorDemeler, Borries
dc.date.accessioned2021-08-19T23:15:52Z
dc.date.available2021-08-19T23:15:52Z
dc.date.issued2017
dc.descriptionAccepted author manuscripten_US
dc.description.abstractInteractions between nucleic acids and proteins are critical for many cellular processes, and their study is of utmost importance to many areas of biochemistry, cellular biology, and virology. Here, we introduce a new analytical method based on sedimentation velocity (SV) analytical ultracentrifugation, in combination with a novel multiwavelength detector to characterize such interactions. We identified the stoichiometry and molar mass of a complex formed during the interaction of a West Nile virus RNA stem loop structure with the human T cell-restricted intracellular antigen-1 related protein. SV has long been proven as a powerful technique for studying dynamic assembly processes under physiological conditions in solution. Here, we demonstrate, for the first time, how the new multiwavelength technology can be exploited to study protein–RNA interactions, and show how the spectral information derived from the new detector complements the traditional hydrodynamic information from analytical ultracentrifugation. Our method allows the protein and nucleic acid signals to be separated by spectral decomposition such that sedimentation information from each individual species, including any complexes, can be clearly identified based on their spectral signatures. The method presented here extends to any interacting system where the interaction partners are spectrally separable.en_US
dc.description.peer-reviewYesen_US
dc.identifier.citationZhang, J., Pearson, J. Z., Gorbet, G. E., Cölfen, H., Germann, M. W. Brinton, M. A., & Demeler, B. (2017). Spectral and hydrodynamic analysis of West-Nile virus RNA-protein interactions by multiwavelength sedimentation velocity in the analytical ultracentrifuge. Analytical Chemistry, 89(1), 862-870. https://doi.org/10.1021/acs.analchem.6b03926en_US
dc.identifier.urihttps://hdl.handle.net/10133/6004
dc.language.isoen_USen_US
dc.publisherAmerican Chemical Societyen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionGeorgia State Universityen_US
dc.publisher.institutionUniversity of Konstanzen_US
dc.publisher.institutionUniversity of Texas Health Science Center at San Antonioen_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.publisher.urlhttps://doi.org/10.1021/acs.analchem.6b03926en_US
dc.subjectSedimentationen_US
dc.subjectQuantum mechanicsen_US
dc.subjectProtein-RNA interactions
dc.subjectAnalytical ultracentrifuges
dc.subject.lcshNucleic acids
dc.subject.lcshHydrodynamics
dc.subject.lcshGenetics
dc.subject.lcshQuantum theory
dc.subject.lcshRNA
dc.subject.lcshWest Nile virus
dc.titleSpectral and hydrodynamic analysis of West Nile virus RNA–protein interactions by multiwavelength sedimentation velocity in the analytical ultracentrifugeen_US
dc.typeArticleen_US
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