Mechanistic characterization of pseudouridine formation by eukaryotic H/ACA snoRNPs

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dc.contributor.authorNamala, Mehar Gayatri Devi
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorKothe, Ute
dc.contributor.supervisorPatel, Trushar R.
dc.date.accessioned2023-03-01T18:27:18Z
dc.date.available2023-03-01T18:27:18Z
dc.date.issued2022
dc.degree.levelPh.Den_US
dc.description.abstractThe H/ACA snoRNP complex directs the formation of pseudouridines in ribosomal RNA (rRNA); however, the mechanisms by which the eukaryotic two-hairpin structured H/ACA snoRNP mediates pseudouridylation in rRNA are poorly understood. This study investigates the function of the conserved two-hairpin structure of eukaryotic H/ACA snoRNA while modifying large, structured rRNA. The biochemical analysis reveals that the two hairpins of the snoRNP complex can independently convert two uridines to two pseudouridines in long rRNA fragments. Moreover, the H/ACA snoRNP can likely unfold secondary structures in rRNA without the help of additional factors. Further, the GAR domains of Gar1 are required for pseudouridine formation, but the KKE/D extensions of Cbf5 play no role in pseudouridylation of rRNA. In summary, this study addresses the mechanism of the H/ACA snoRNP complex in modifying rRNA during ribosome biogenesis.en_US
dc.identifier.urihttps://hdl.handle.net/10133/6443
dc.language.isoenen_US
dc.proquestyesNoen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)
dc.subjectRNA
dc.subjectbio-molecular sciences
dc.subjectbiochemistry
dc.subjectRNA--Research
dc.subjectBiochemistry
dc.subjectPseudouridine--Research
dc.subjectNon-coding RNA--Research
dc.subjectMolecular biology
dc.subjectEukaryotic cells
dc.subjectDissertations
dc.subjectAcademic
dc.titleMechanistic characterization of pseudouridine formation by eukaryotic H/ACA snoRNPsen_US
dc.typeThesis
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