The role of tetracycline and macromolecular crowding on the function of elongation factor-Tu

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dc.contributor.authorGzyl, Katherine Elizabeth
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorWieden, Hans-Joachim
dc.date.accessioned2018-04-12T23:47:55Z
dc.date.available2018-04-12T23:47:55Z
dc.date.issued2017
dc.degree.levelMastersen_US
dc.description.abstractThe cellular environment affects dynamics of proteins moving from one conformation into another. Aspects of the cellular environment such as availability of ligands, osmolytes, and antibiotics affect proteins. However, the effect of macromolecular crowding remains debated. The translational GTPase EF-Tu undergoes a large conformational change during its delivery of aa-tRNA to the ribosome. Here, we investigated whether the antibiotic tetracycline or the crowded nature of the cell has an affect on the dynamics of EF-Tu. By comparing the rates of nucleotide binding, the GTPase activity of EF-Tu, and the stability of the EF-Tu- GTP -aa-tRNA complex with and without tetracycline, it was concluded that tetracycline did not affect the function of EF-Tu. Macromolecular crowding by the synthetic crowding agents dextran 40 and PEG 4000 was found to influence the dynamics of nucleotide binding in EF-Tu suggesting the dynamics and function of EF-Tu should be studied in a more cellular-like context.en_US
dc.embargoNoen_US
dc.identifier.urihttps://hdl.handle.net/10133/5087
dc.language.isoen_USen_US
dc.proquest.subject0379en_US
dc.proquest.subject0307en_US
dc.proquest.subject0487en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : Universtiy of Lethbridge, Department of Chemistry and Biochemistryen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectTetracyclinesen_US
dc.subjectGenetic translation--Researchen_US
dc.subjectG proteinsen_US
dc.subjectProteins--Synthesis--Inhibitorsen_US
dc.subjectbacterial translationen_US
dc.subjectelongation factor-Tuen_US
dc.subjectmacromolecular crowdingen_US
dc.subjectnucleotide bindingen_US
dc.subjectsmall molecule inhibitorsen_US
dc.titleThe role of tetracycline and macromolecular crowding on the function of elongation factor-Tuen_US
dc.typeThesisen_US
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