Overexpression of Douglas-Fir LEAFY COTYLEDON1 (PmLEC1) in Arabidopsis induces embryonic programs and embryo-like structures in the lec1-1 mutant but not in wild type plants

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dc.contributor.authorVetrici, Mariana A.
dc.contributor.authorYevtushenko, Dmytro P.
dc.contributor.authorMisra, Santosh
dc.date.accessioned2024-09-26T22:14:04Z
dc.date.available2024-09-26T22:14:04Z
dc.date.issued2021
dc.descriptionOpen access article. Creative Commons Attribution 4.0 International license (CC BY 4.0) applies
dc.description.abstractSomatic embryogenesis (SE) is the most promising method for the quick propagation of desirable plant genotypes. However, application of SE to conifers remains challenging due to our limited knowledge about the genes involved in embryogenesis and the processes that lead to somatic embryo formation. Douglas-fir, an economically important lumber species, possesses a homolog of the angiosperm embryo-regulatory LEC1 gene. In the present study, we analyzed the potential of Douglas-fir PmLEC1 to induce embryonic programs in the vegetative cells of a heterologous host, Arabidopsis thaliana. PmLEC1 complemented the Arabidopsis lec1-1 null mutant and led to a variety of phenotypes ranging from normal morphology to developmental arrest at various stages in the T1 generation. PmLEC1 did not affect the morphology of wild type Arabidopsis T1 plants. More profound results occurred in T2 generations. PmLEC1 expression induced formation of recurrent somatic embryo-like structures in vegetative tissues of the rescued lec1-1 mutant but loss of apical dominance (bushy phenotype) in wild type plants. The activation of embryonic programs in the lec1-1PmLEC1 T2 plants was confirmed by the presence of the embryo-specific transcripts, OLEOSIN and CRUCIFERIN. In contrast, no embryo-like structures, and no OLEOSIN or CRUCIFERIN were observed in PmLEC1-expressing bushy wild type T2 plants.
dc.description.peer-reviewYes
dc.identifier.citationVetrici, M. A., Yevtushenko, D. P., & Misra, S. (2021). Overexpression of Douglas-Fir LEAFY COTYLEDON1 (PmLEC1) in Arabidopsis induces embryonic programs and embryo-like structures in the lec1-1 mutant but not in wild type plants . Plants, 10(8), Article 1526. https://doi.org/10.3390/plants10081526
dc.identifier.urihttps://hdl.handle.net/10133/6920
dc.language.isoen
dc.publisherMDPI
dc.publisher.departmentDepartment of Biological Science
dc.publisher.facultyArts and Science
dc.publisher.institutionUniversity of Lethbridge
dc.publisher.institutionUniversity of Victoria
dc.publisher.urlhttps://doi.org/10.3390/plants10081526
dc.subjectDouglas-fir
dc.subjectSomatic embryogenesis
dc.subjectLEAFY COTYLEDON1
dc.subjectTransformation
dc.subjectLec1-1 null mutant
dc.subjectEmbryo-specific gene expression
dc.subjectAnti-PmLEC1 antibodies
dc.subject.lcshDouglas fir--Propagation
dc.titleOverexpression of Douglas-Fir LEAFY COTYLEDON1 (PmLEC1) in Arabidopsis induces embryonic programs and embryo-like structures in the lec1-1 mutant but not in wild type plants
dc.typeArticle
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