Longitudinal characterization of antimicrobial resistance genes in feces shed from cattle fed different subtherapeutic antibiotics

Simple item page
dc.contributor.authorAlexander, Trevor W.
dc.contributor.authorYanke, L. Jay
dc.contributor.authorReuter, Tim
dc.contributor.authorTopp, Ed
dc.contributor.authorRead, Ronald R.
dc.contributor.authorSelinger, L. Brent
dc.contributor.authorMcAllister, Tim A.
dc.date.accessioned2017-03-17T18:04:16Z
dc.date.available2017-03-17T18:04:16Z
dc.date.issued2011
dc.descriptionSherpa Romeo green journal. Open access article. Creative Commons Attribution 2.0 Generic License (CC BY 2.0) appliesen_US
dc.description.abstractBackground: Environmental transmission of antimicrobial-resistant bacteria and resistance gene determinants originating from livestock is affected by their persistence in agricultural-related matrices. This study investigated the effects of administering subtherapeutic concentrations of antimicrobials to beef cattle on the abundance and persistence of resistance genes within the microbial community of fecal deposits. Cattle (three pens per treatment, 10 steers per pen) were administered chlortetracycline, chlortetracycline plus sulfamethazine, tylosin, or no antimicrobials (control). Model fecal deposits (n = 3) were prepared by mixing fresh feces from each pen into a single composite sample. Real-time PCR was used to measure concentrations of tet, sul and erm resistance genes in DNA extracted from composites over 175 days of environmental exposure in the field. The microbial communities were analyzed by quantification and denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S-rRNA. Results: The concentrations of 16S-rRNA in feces were similar across treatments and increased by day 56, declining thereafter. DGGE profiles of 16S-rRNA differed amongst treatments and with time, illustrating temporal shifts in microbial communities. All measured resistance gene determinants were quantifiable in feces after 175 days. Antimicrobial treatment differentially affected the abundance of certain resistance genes but generally not their persistence. In the first 56 days, concentrations of tet(B), tet(C), sul1, sul2, erm(A) tended to increase, and decline thereafter, whereas tet(M) and tet(W) gradually declined over 175 days. At day 7, the concentration of erm(X) was greatest in feces from cattle fed tylosin, compared to all other treatments. Conclusion: The abundance of genes coding for antimicrobial resistance in bovine feces can be affected by inclusion of antibiotics in the feed. Resistance genes can persist in feces from cattle beyond 175 days with concentrations of some genes increasing with time. Management practices that accelerate DNA degradation such as frequent land application or composting of manure may reduce the extent to which bovine feces serves as a reservoir of antimicrobial resistance.en_US
dc.description.peer-reviewYesen_US
dc.identifier.citationAlexander, T.W., Yanke, J. L., Reuter, T., Topp, E., Read, R. R., Selinger, L. B., & McAllister, T. A. (2011). Longitudinal characterization of antimicrobial resistance genes in feces shed from cattle fed different subtherapeutic antibiotics. BMC Microbiology, 11:19. doi:10.1186/1471-2180-11-19en_US
dc.identifier.urihttps://hdl.handle.net/10133/4798
dc.language.isoen_USen_US
dc.publisherBiomed Central Ltden_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionUniversity of Vermonten_US
dc.publisher.institutionLethbridge Research Centreen_US
dc.publisher.institutionAgriculture and Agri-Food Research Centre (Ont.)en_US
dc.publisher.institutionUniversity of Calgaryen_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.subjectAntimicrobial resistanceen_US
dc.subjectBovine fecesen_US
dc.subjectAntibioticsen_US
dc.subjectBeef cattleen_US
dc.subjectAntibiotics in animal nutritionen_US
dc.titleLongitudinal characterization of antimicrobial resistance genes in feces shed from cattle fed different subtherapeutic antibioticsen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Selinger longitudinal characterization of antimicrobial.pdf
Size:
1 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Selinger, Brent