In vitro evaluation of linear plant host defense peptides Shepherin 2, Skh-AMP1, Cn-AMP1, and Cr-ACP1 for cytotoxicity and antimicrobial activities against potato pathogens

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dc.contributor.authorStephen, Christie M.
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorYevtushenko, Dmytro P.
dc.date.accessioned2023-08-01T15:51:30Z
dc.date.available2023-08-01T15:51:30Z
dc.date.issued2023
dc.degree.levelMasters
dc.description.abstractThe aim of this study was to evaluate the antimicrobial and cytotoxic activities of four linear plant host defense peptides (HDPs), Shepherin 2, Cr-ACP1, Skh-AMP1, and Cn-AMP1, in vitro and to identify potential candidates for transgenic expression in potato plants for enhanced disease resistance. The antimicrobial activities of the HDPs were tested against major potato pathogens: the fungi Fusarium culmorum and Alternaria solani, and the bacteria Pectobacterium carotovorum and P. atrosepticum. Shepherin 2 had the strongest antimicrobial activity, inhibiting the germination of 98% F. culmorum conidia at 16 µM, and 84% P. carotovorum cells at 64 µM. Combination of Shepherin 2 with Cr-ACP1 resulted in inhibition of 89% P. carotovorum cells over the expected 70% at 16μM, indicating a synergistic interaction. All HDPs had low cytotoxicities toward plant protoplasts and mammalian cells. The gene encoding Shepherin 2 was introduced into potato plants for future evaluation of its activity in planta.
dc.identifier.urihttps://hdl.handle.net/10133/6548
dc.language.isoen
dc.proquest.subject0285
dc.proquest.subject0479
dc.proquest.subject0307
dc.proquestyesYes
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences
dc.publisher.departmentDepartment of Biological Sciences
dc.publisher.facultyArts and Science
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)
dc.subjectlinear plant host defense peptides
dc.subjectcytotoxicity activity
dc.subjectantimicrobial activity
dc.subjectpotato pathogens
dc.subjectShepherin 2
dc.subjectCr-ACP1
dc.subjectSkh-AMP1
dc.subjectCn-AMP1
dc.subject.lcshPlants--Disease and pest resistance--Research
dc.subject.lcshPlants--Disease and pest resistance--Genetic aspects--Research
dc.subject.lcshPlants--Disease and pest resistance--Molecular aspects--Research
dc.subject.lcshPotatoes--Disease and pest resistance--Research
dc.subject.lcshPeptides--Biotechnology--Research
dc.subject.lcshPlant genetic engineering
dc.subject.lcshPlant molecular biology
dc.subject.lcshPlant-pathogen relationships
dc.subject.lcshDissertations, Academic
dc.titleIn vitro evaluation of linear plant host defense peptides Shepherin 2, Skh-AMP1, Cn-AMP1, and Cr-ACP1 for cytotoxicity and antimicrobial activities against potato pathogens
dc.typeThesis
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