rpsA and ribosomal protein S1: investigating a non-canonical translation initiation element

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dc.contributor.authorVigar, Justin R. J.
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorWieden, Hans-Joachim
dc.date.accessioned2020-01-22T20:51:05Z
dc.date.available2020-01-22T20:51:05Z
dc.date.issued2019
dc.degree.levelMastersen_US
dc.description.abstractTranslation initiation rates are fine-tuned by altering interactions between the ribosome and the translation initiation region of an mRNA. In bacteria varying levels of RNA structure attenuate these interactions by masking the ribosome-binding site from the small ribosomal subunit. Recent studies have described diverse strategies for recruiting mRNA to the ribosome, and highlighted the contributions of ribosomal protein S1. Here, I provide evidence that the non-canonical initiation mechanism that governs translation of the rpsA mRNA, encoding ribosomal protein S1, contains a three-dimensional architecture that is required for efficient translation. Furthermore, S1 plays an essential role during the initiation phase of translation by recruiting mRNA to the ribosome—unfolding structured mRNAs, and allowing for correct start codon positioning on the ribosome. Combining crosslinking immunoprecipitation and high-throughput sequencing approaches revealed the extent of S1’s involvement in mRNA recruitment, while also highlighting a broader role as a regulator of many RNA classes.en_US
dc.description.sponsorshipAlberta Innovates (Strategic Chairs Program) [SC60-T2]en_US
dc.identifier.urihttps://hdl.handle.net/10133/5670
dc.language.isoen_USen_US
dc.proquest.subject0487en_US
dc.proquest.subject0786en_US
dc.proquest.subject0537en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Department of Chemistry and Biochemistryen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectRNAen_US
dc.subjectBiophysicsen_US
dc.subjectSynthetic Biologyen_US
dc.subjectRibosomeen_US
dc.subjectTranslation Initiationen_US
dc.subjectEngineeringen_US
dc.subjectRNA-seqen_US
dc.subjectDissertations, Academicen_US
dc.titlerpsA and ribosomal protein S1: investigating a non-canonical translation initiation elementen_US
dc.typeThesisen_US
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