Two partners of the ribosome, EF-Tu and LepA

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dc.contributor.authorDe Laurentiis, Evelina I.
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorWieden, Hans-Joachim
dc.date.accessioned2011-08-19T19:21:14Z
dc.date.available2011-08-19T19:21:14Z
dc.date.issued2009
dc.degree.levelMasters
dc.descriptionxii, 127 leaves : ill. (some col.) ; 29 cmen_US
dc.description.abstractThe translational GTPases elongation factor Tu (EF-Tu) and LepA modulate the dynamics of tRNA on the ribosome. EF-Tu facilitates the delivery of aminoacyl-tRNA (aa-tRNA) to the translating ribosome and LepA catalyzes the retro-translocation of tRNA•mRNA from the E- and P-sites of the ribosome back to the P- and A-sites. Although an increasing body of structural and biochemical information is available, little is known about the functional cycle of LepA during retro-translocation, the kinetics of EF-Tu dissociation from the ribosome and the rate of EF-Tu conformational change during aa-tRNA delivery. This thesis reports the successful construction and biochemical characterisation of a mutant form of EF-Tu from Escherichia coli ideal for the specific incorporation of fluorescent labels, enabling measurements pivotal for uncovering the rate of EF-Tu conformational change and dissociation from the ribosome. Furthermore, to determine structural components critical for LepA’s function, mutant versions of the protein were constructed and biochemically characterised.en_US
dc.identifier.urihttps://hdl.handle.net/10133/2515
dc.language.isoen_USen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry, c2009en_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectSignal peptidasesen_US
dc.subjectRibosomes -- Structureen_US
dc.subjectProteins -- Synthesisen_US
dc.subjectG proteinsen_US
dc.subjectDissertations, Academicen_US
dc.titleTwo partners of the ribosome, EF-Tu and LepAen_US
dc.typeThesisen_US
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