Genome-wide profiling of transfer RNAs and their role as novel prognostic markers for breast cancer

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dc.contributor.authorKrishnan, Preethi
dc.contributor.authorGhosh, Sunita
dc.contributor.authorWang, Bo
dc.contributor.authorHeyns, Mieke
dc.contributor.authorLi, Dongping
dc.contributor.authorMackey, John R.
dc.contributor.authorKovalchuk, Olga
dc.contributor.authorDamaraju, Sambasivarao
dc.date.accessioned2017-07-13T21:51:17Z
dc.date.available2017-07-13T21:51:17Z
dc.date.issued2016
dc.descriptionSherpa Romeo green journal: open accessen_US
dc.description.abstractTransfer RNAs (tRNAs, key molecules in protein synthesis) have not been investigated as potential prognostic markers in breast cancer (BC), despite early findings of their dysregulation and diagnostic potential. We aim to comprehensively profile tRNAs from breast tissues and to evaluate their role as prognostic markers (Overall Survival, OS and Recurrence Free Survival, RFS). tRNAs were profiled from 11 normal breast and 104 breast tumor tissues using next generation sequencing. We adopted a Case-control (CC) and Case-Only (CO) association study designs. Risk scores constructed from tRNAs were subjected to univariate and multivariate Cox-proportional hazards regression to investigate their prognostic value. Of the 571 tRNAs profiled, 76 were differentially expressed (DE) and three were significant for OS in the CC approach. We identified an additional 11 tRNAs associated with OS and 14 tRNAs as significant for RFS in the CO approach, indicating that CC alone may not capture all discriminatory tRNAs in prognoses. In both the approaches, the risk scores were significant in the multivariate analysis as independent prognostic factors, and patients belonging to high-risk group were associated with poor prognosis. Our results confirmed global up-regulation of tRNAs in BC and identified tRNAs as potential novel prognostic markers for BC.en_US
dc.description.peer-reviewYesen_US
dc.identifier.citationKrishnan, P., Ghosh, S., Wang, B., Heyns, M., Li, D., Mackey, J.R. ...Damaraju, S. (2016). Genome-wide profling of transfer RNAs and their role as novel prognostic markers for breast cancer. Scientific Report, 6, 32843. doi:10.1038/srep32483(2016)en_US
dc.identifier.urihttps://hdl.handle.net/10133/4867
dc.language.isoen_USen_US
dc.publisherNature Publishing Groupen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionUniversity of Albertaen_US
dc.publisher.institutionCross Cancer Instituteen_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.subjectTransfer RNAsen_US
dc.subjectPrognostic markeren_US
dc.subjectOverall survivalen_US
dc.subjectRecurrence free survivalen_US
dc.subjectBreast canceren_US
dc.subjectNext generation sequencingen_US
dc.titleGenome-wide profiling of transfer RNAs and their role as novel prognostic markers for breast canceren_US
dc.typeArticleen_US
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