Gene expression in a microspore-derived cell suspension culture of Brassica Napus exhibiting enhanced oil production

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dc.contributor.authorDavoren, Jonathan M.
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorWeselake, Randall
dc.contributor.supervisorLaroche, Andre
dc.date.accessioned2007-07-12T20:20:51Z
dc.date.available2007-07-12T20:20:51Z
dc.date.issued1997
dc.degree.levelMasters
dc.descriptionxxi, 256 leaves : ill. ; 28 cm.en
dc.description.abstractTriacylglycerol (TAG) production in the microspore derived (MD) cell suspension culture ofBrassica napus L. cv Jet Neuf was enhanced when the sucrose concentration in the growth medium was increased from 2 to 14 % (w/v). mRNA differential display by polymerase chain reaction was used to examine gene expression in cells grown at different sucrose concentrations in order to identify mRNAs which could be associated with oil formation. The anchored primer, T12AA, was used to screen one subset, representing approximately one twelfth of the transcript population, isolated from cultures grown in media supplemented to 2, 6 and 14 % (w/v) sucrose. Analysis of this mRNA subset revealed thirteen cDNAs which appeared to be upregulated as the sucrose concentration was increased. Cloning and sequencing revealed multiple cDNA fragments for each signal detected by differential display. RT-PCR analysis of sixteen different cDNAs revealed that eight encoded mRNAs which were upregulated in parallel to the increase in media sucrose. Comparison of the eight upregulated cDNAs to other sequences in GenBank revealed the following: (1) BSS8A had a 100% identity with the last 25 amino acids of an acyl carrier protein from Arabidopsis thaliana, (2) BSS1A displayed homology to a number of sequences of unknown function, (3) BSS1 IB displayed weak but significant homology to a number of sequences of unknown function, (4) BSS13A displayed homology to four members of the thioredoxin family from ,4. thaliana and (5) four Had no significant homology to previously reported sequences which makes them potential candidates to encode lipogenic enzymes. These results indicate that differential display of mRNA may be a simple and rapid method for the identification of sucrose-modulated gene expression changes in this system and for the characterization of novel sequences potentially encoding lipogenic proteins.en
dc.identifier.urihttps://hdl.handle.net/10133/345
dc.language.isoen_USen
dc.publisherLethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 1997en
dc.publisher.departmentDepartment of Chemistry and Biochemistryen
dc.publisher.facultyArts and Scienceen
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en
dc.subjectDissertations, Academicen
dc.subjectRape (Plant) -- Researchen
dc.subjectOilseed plants -- Researchen
dc.subjectGene expressionen
dc.titleGene expression in a microspore-derived cell suspension culture of Brassica Napus exhibiting enhanced oil productionen
dc.typeThesisen
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