Distinguishing features of guide and substrate RNA recognition by H/ACA snoRNPs

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dc.contributor.authorKelly, Erin Katelyn
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorWieden-Kothe, Ute
dc.date.accessioned2018-11-26T17:49:17Z
dc.date.available2018-11-26T17:49:17Z
dc.date.issued2018
dc.degree.levelMastersen_US
dc.description.abstractH/ACA small nucleolar ribonucleoproteins (snoRNPs) pseudouridylate RNA in eukaryotes and archaea. They consist of four proteins and a guide RNA. It is unclear which element of the guide RNA the H/ACA proteins recognize. By designing guide RNA variants with altered structural features, I determined that H/ACA proteins bind guide RNA extremely tightly, but non-specifically in vitro. Additionally, the base-pairing between guide and substrate RNA varies widely in nature. To elucidate substrate selection rules, I systematically altered the sequence of a substrate RNA and tested for snoRNP binding and activity. All substrate RNA variants tested are bound by the H/ACA snoRNP, but not all are effectively modified. Minimal continuous base pairing is required to position the target uridine in the active site for modification, and H/ACA snoRNPs can target structured RNAs. This information will improve the prediction of novel target sites of H/ACA snoRNPs, e.g. in messenger RNAs.en_US
dc.description.sponsorshipNSERCen_US
dc.embargoNoen_US
dc.identifier.urihttps://hdl.handle.net/10133/5247
dc.language.isoen_USen_US
dc.proquest.subject0307en_US
dc.proquest.subject0379en_US
dc.proquest.subject0786en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : Universtiy of Lethbridge, Department of Chemistry and Biochemistryen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectRNA modificationen_US
dc.subjectH/ACA snoRNPen_US
dc.subjectSaccharomyces cerevisiaeen_US
dc.subjectguide RNAen_US
dc.subjectsubstrate RNAen_US
dc.subjectPseudouridineen_US
dc.subjectNucleoproteinsen_US
dc.subjectNon-coding RNAen_US
dc.subjectMolecular biologyen_US
dc.subjectDissertations, Academicen_US
dc.titleDistinguishing features of guide and substrate RNA recognition by H/ACA snoRNPsen_US
dc.typeThesisen_US
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